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Product Name phospho-IKB epsilon (Ser22) Chinese Name NF-kappa-B抑制子epsilon抗体 Alias IKB epsilon (phospho S22); p-IKB epsilon (phospho S22); I kappa B epsilon; I-kappa-B-epsilon; IkappaBepsilon; IkB E; IkB-E; IkB-epsilon; IKBE; IKBE_MOUSE; MGC72568; NF kappa B inhibitor epsilon; NF kappa BIE; NF-kappa-B inhibitor epsilon; NF-kappa-BIE; NFkappa BIE; NFkappaB inhibitor epsilon; NFKBIE; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor epsilon; OTTHUMP00000016522; Slc35b2; solute carrier family 35, member B2. Research Area Cell biology immunology Chromatin and nuclear signals Signal transduction transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Cow, Sheep, ) Applications WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 39kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human IKB epsilon around the phosphorylation site of Ser22: LR(p-S)LR Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011]
Function:
Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
Subunit:
Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit.
Subcellular Location:
Cytoplasm.
Post-translational modifications:
Serine phosphorylated; followed by proteasome-dependent degradation.
Similarity:
Belongs to the NF-kappa-B inhibitor family.
Contains 6 ANK repeats.
SWISS:
O00221
Gene ID:
4794
Database links:Entrez Gene: 4794 Human
Entrez Gene: 18037 Mouse
Omim: 604548 Human
SwissProt: O00221 Human
SwissProt: O54910 Mouse
Unigene: 458276 Human
Unigene: 57043 Mouse
Product Picture Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-phospho-IKB epsilon (Ser22) (SL15582R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IKB epsilon (Ser22)) Polyclonal Antibody, Unconjugated (SL15582R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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