Product Name	OCLN
Chinese Name	紧密连接蛋白抗体
Alias	OCLN_HUMAN; Occludin; Ocl; BLCPMG; PPP1R115; PTORCH1; Tight junction protein occludin;
literatures	Specific References  (18)     |     SL1495R has been referenced in 18 publications. [IF=2.984]   WB, IF ;  Pig.   PubMed:32717305 [IF=15.923] Changsheng Xing. et al. Microbiota regulate innate immune signaling and protective immunity against cancer. Cell Host Microbe. 2021 Apr;:  IHC ;  Mouse.   PubMed:33894128 [IF=8.039] Yifan Zhu. et al. Discovery of Selective P2Y6R Antagonists with High Affinity and In Vivo Efficacy for Inflammatory Disease Therapy. J MED CHEM. 2023;XXXX(XXX):XXX-XXX  WB ;  Mouse.   PubMed:37078976 [IF=4.122] Hayes CL.et al. Commensal microbiota induces colonic barrier structure and functions that contribute to homeostasis. (2018)Scientific Reports, 8(1).  IF ;  Mouse.   PubMed:30242285 [IF=4.12] Zou et al. Huangqin-tang ameliorates dextran sodium sulphate-induced colitis by regulating intestinal epithelial cell homeostasis, inflammation and immune response. (2016) Sci.Rep. 6:39299  IHC ;  Mouse.   PubMed:27982094 [IF=3.743] Li N et al. Modification effects of SanWei GanJiang Powder on liver and intestinal damage through reversing bile acid homeostasis. Biomed Pharmacother. 2019 Aug;116:109044.  IHC-P ;  Mouse.   PubMed:31174088 [IF=3.545] Lackner I et al. Toll-Like Receptor-Mediated Cardiac Injury during Experimental Sepsis. Mediators of Inflammation, 2020, 1–12.  ICF ;  Mouse.   PubMed:doi:10.1155/2020/6051983 [IF=3.47] Li T et al. Milk fat globule membrane supplementation modulates the gut microbiota and attenuates metabolic endotoxemia in high-fat diet-fed mice. Journal of Functional Foods,2018 47, 56–65.  WB ;  Mouse.   PubMed:10.1016/j.jff.2018.05.038 [IF=3.454] Li T et al. Bovine α-lactalbumin hydrolysates ameliorate obesity-associated endotoxemia and inflammation in high-fat diet-fed mice through modulation of gut microbiota. Food Funct. 2019 May 17.  WB ;  Mouse.   PubMed:31099356 [IF=2.984] Ruirui Luoet al. Clostridium perfringens beta2 toxin induced in vitro oxidative damage and its toxic assessment in porcine small intestinal epithelial cell lines. Gene . 2020 Oct 30;759:144999.  IF ;  pig.   PubMed:32717305 [IF=2.86] Zhao, Yuan, et al. "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets."International journal of molecular sciences 12.12 (2011): 8502-8512.  WB, IHC-P ;  Pig.   PubMed:22272087 [IF=2.776] Weber B et al. Lung injury after asphyxia and hemorrhagic shock in newborn piglets: Analysis of structural and inflammatory changes. PLoS One. 2019 Jul 5;14(7):e0219211.  IHF ;  Piglet.   PubMed:31276543 [IF=2.73] Sun, Yan‐Na, et al. "Effects of insulin combined with idebenone on blood–brain barrier permeability in diabetic rats." Journal of Neuroscience Research (2014).  WB ;  Rat.   PubMed:25421718 [IF=2.71] Guo, Xiaoling, et al. "Matrigel and Activin A promote cell-cell contact and anti-apoptotic activity in cultured human retinal pigment epithelium cells." Experimental Eye Research (2016).  IF(ICC) ;  Human.   PubMed:27130547 [IF=2.46] Zhao, Yuan, et al. "β-Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2." International Journal of Molecular Sciences 15.2 (2014): 1915-1926.  Pig.   PubMed:24473141 [IF=2.46] Pan, Li, et al. "Effects of Soybean Agglutinin on Mechanical Barrier Function and Tight Junction Protein Expression in Intestinal Epithelial Cells from Piglets." International Journal of Molecular Sciences 14.11 (2013): 21689-21704.  Pig.   PubMed:24189218 [IF=2.03] Gu, Yan-ting, et al. "Calcium-activated potassium channel activator down-regulated the expression of tight junction protein in brain tumor model in rats." Neuroscience letters 493.3 (2011): 140-144.  WB ;  Rat.   PubMed:21334421 [IF=0] Yılmaz Ö et al. May zonula occludens proteins regulate the pathogenesis of allergic rhinitis? Tr-ENT 2019;29(2):100-106.  IHC-P ;  Human.   PubMed:Doi:10.5606/Tr-ENT.2019.64936
Research Area	Cell biology  immunology  transcriptional regulatory factor  Cell adhesion molecule
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=2µg/Test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	57kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from mouse OCLN: 431-522/522 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011] Function: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. Subunit: Interacts with TJP1/ZO1 and with VAPA. Subcellular Location: Membrane; Multi-pass membrane protein. Cell junction, tight junction. Tissue Specificity: Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. May be phosphorylated by PKC during translocation to cell-cell contacts. Similarity: Belongs to the ELL/occludin family. Contains 1 MARVEL domain. SWISS: Q61146 Gene ID: 18260 Database links: Entrez Gene: 18260 Mouse Entrez Gene: 100506658 Human Entrez Gene: 397236 Pig Entrez Gene: 83497 Rat Entrez Gene: 403844 Dog Omim: 602876 Human SwissProt: Q28269 Dog SwissProt: Q16625 Human SwissProt: Q61146 Mouse SwissProt: Q6P6T5 Rat Unigene: 592605 Human Unigene: 4807 Mouse Unigene: 31429 Rat
Product Picture	Sample: A549 Cell Lysate at 30 ug Primary: Anti- OCLN (SL1495R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug Lane 2: MCF-7 (Human) Cell Lysate at 30 ug Lane 3: A431 (Human) Cell Lysate at 30 ug Lane 4: HepG2 (Human) Cell Lysate at 30 ug Lane 5: Hela (Human) Cell Lysate at 30 ug Primary: Anti-OCLN (SL1495R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65/55/38/28 kD Observed band size: 65/38/28 kD Sample: A549 Cell Lysate at 30 ug Primary: Anti- OCLN (SL1495R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 60 kD Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-OCLN (SL1495R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 57 kD Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-OCLN antibody (SL1495R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. The blue histogram is unstained cells (293FT cells). The Wathet Blue histogram is cells stained with secondary antibody alone. The Orange histogram is cells stained with rabbit IgG isotype control antibody plus secondary antibody. The green histogram is cells stained with Rabbit Anti-OCLN antibody (SL1495R) plus secondary antibody (Concentraion 1: 20).

Cartpieces

• 
• 1

  1Delete

• 
• 5

  5Delete

• 
• S

  SDelete

• 
• R

  RDelete

• 
• +

  +Delete

• 
• 4

  4Delete

• Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• O

  ODelete

• 
• O

  ODelete

• 

• Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• R

  RDelete

• 
• 5

  5Delete

• 
• A

  ADelete

Totalgoods,subtotals:￥Checkout