Product Name	PLA2G7
Chinese Name	Lipoprotein磷脂酶A2抗体
Alias	Lp-PLA2; PAFAH; lipoprotein-associated phospholipase A2; Platelet-activating factor acetylhydrolase; Lipoprotein-associated phospholipaseA2 (Lp-PLA2); PAF acetylhydrolase; PAF 2-acylhydrolase; LDL-associated phospholipase A2; LDL-PLA(2); 2-acetyl-1-alkylglycerophosphocholine esterase; 1-alkyl-2-acetylglycerophosphocholine esterase; PAFA_HUMAN.  Lipoprotein相关磷脂酶A2;
literatures	Specific References  (4)     |     SL1451R has been referenced in 4 publications. [IF=3.71] Rosing, Katharina, et al. Everolimus therapy is associated with reduced lipoprotein-associated phospholipase A2 (Lp-Pla2) activity and oxidative stress in heart transplant recipients. Atherosclerosis. 2013 Sep;230(1):164-70.  WB ;  Human.   PubMed:23958269 [IF=2.93] Wang, Gangqi, et al. "Nitro-oleic acid downregulates lipoprotein-associated phospholipase A2 expression via the p42/p44 MAPK and NF [kgr] B pathways." Scientific Reports 4 (2014).  WB ;  Human.   PubMed:24809325 [IF=2.1] Han et al. Apolipoprotein CIII regulates lipoprotein-associated phospholipase A2 expression via the MAPK and NFκB pathways. (2015) Biol.Open. 4:661-5  WB ;  Porcine.   PubMed:25836672 [IF=0] Wihastuti TA et al. Decreasing angiogenesis vasa vasorum through Lp-PLA2 and H2O2 inhibition by PSP from Ganoderma lucidum in atherosclerosis: in vivo diabetes mellitus type 2. J Basic Clin Physiol Pharmacol. 2020 Feb 7;30(6).  IHC ;  Rat.   PubMed:32031980
Research Area	Cardiovascular  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	48kDa
Cellular localization	Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PAFAH: 301-441/441
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes platelet-activating factor acetylhydrolase isoform 2, a single-subunit intracellular enzyme that catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl -sn-glyceryl-3-phosphorylcholine). However, this lipase exhibits a broader substrate specificity than simply platelet activating factor. Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist, and both are multi-subunit enzymes. Additionally, there is a single-subunit serum isoform of this enzyme. Function: Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. Subcellular Location: Secreted, extracellular space. Tissue Specificity: Plasma. DISEASE: Platelet-activating factor acetylhydrolase deficiency (PAFAD) [MIM:614278]: An enzymatic deficiency that results in exacerbated bodily response to inflammatory agents. It can be associated with several disease states including inflammatory gastrointestinal disorders, asthma and atopy. Asthmatic individuals with PAFAD may manifest aggravated respiratory symptoms. Note=The disease is caused by mutations affecting the gene represented in this entry. Asthma (ASTHMA) [MIM:600807]: The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. Atopic hypersensitivity (ATOPY) [MIM:147050]: A condition characterized by predisposition to develop hypersensitivity reactions. Atopic individuals can develop eczema, allergic rhinitis and allergic asthma. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. Similarity: Belongs to the AB hydrolase superfamily. Lipase family. SWISS: Q13093 Gene ID: 7941 Database links: Entrez Gene: 7941 Human Omim: 601690 Human SwissProt: Q13093 Human Lipoprotein相关磷脂A2（Lp-PLA2）也称血浆血小板激活因子乙酰水解酶，是介导氧化低密度Lipoprotein引起炎症和动脉粥样硬化的一个关键性酶。 Lipoprotein相关磷脂酶A2是磷脂酶A2超家族的一个成员,由成熟的巨噬细胞和lymphocyte合成和分泌,主要与低密度Lipoprotein结合,能水解低密度Lipoprotein上的氧化卵磷脂,生成促炎物质溶血卵磷脂和氧化型游离脂肪酸,促进动脉粥样硬化形成,经研究认为：抑制Lipoprotein相关磷脂酶A2活性可能有抗动脉粥样硬化的效应。
Product Picture	Sample: Lung (Rat) Lysate at 40 ug Primary: Anti-PAFAH (SL1451R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAFAH) Polyclonal Antibody, Unconjugated (SL1451R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAFAH) Polyclonal Antibody, Unconjugated (SL1451R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PAFAH2/Lp-PLA2 Polyclonal Antibody, Unconjugated(SL1451R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat spleen tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PAFAH2 Polyclonal Antibody, Unconjugated(SL1451R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.

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