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Product Name B7-H6 Chinese Name B7H6蛋白抗体 Alias B7 homolog 6; B7-H6; B7H6; B7H6_HUMAN; DKFZp686O24166; Natural cytotoxicity triggering receptor 3 ligand 1; NCR3LG1; Putative Ig like domain containing protein DKFZp686O24166/DKFZp686I21167. Research Area Cell biology immunology Chromatin and nuclear signals Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 48kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human B7-H6: 51-150/454 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail B7H6 belongs to the B7 family (see MIM 605402) and is selectively expressed on tumor cells. Interaction of B7H6 with NKp30 (NCR3; MIM 611550) results in natural killer (NK) cell activation and cytotoxicity (Brandt et al., 2009 [PubMed 19528259]).[supplied by OMIM, Jan 2011].
Function:
Triggers NCR3-dependent natural killer cell activation.
Subunit:
Monomer. Interacts specifically with NCR3, but not with other natural killer cell-activating receptors, including NCR1, NCR2 and KLRK1.
Subcellular Location:
Cell membrane; Single-pass type I membrane protein.
Tissue Specificity:
Not detected in any normal tissue tested. Expressed at the surface of several tumor cell lines including T and B lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells (at protein level).
Similarity:
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
SWISS:
Q68D85
Gene ID:
374383
Database links:Entrez Gene: 374383 Human
Omim: 613714 Human
SwissProt: Q68D85 Human
Unigene: 146274 Human
Product Picture Sample:
Heart (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti- B7-H6 (SL12559R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD
Sample:
Huh7 Cell (Human) Lysate at 40 ug
A549 Cell (Human) Lysate at 40 ug
Primary: Anti-B7-H6 (SL12559R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Sample:
Cerebral cortex (Mouse) Lysate at 40 ug
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti- B7-H6 (SL12559R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B7-H6) Polyclonal Antibody, Unconjugated (SL12559R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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