Product Name	NFIB
Chinese Name	核因子1B抗体
Alias	CCAAT Box Binding Transcription Factor; CCAAT-box-binding transcription factor; CTF; NF-I/B; NF1-B; NF1B; NF1B2; NFI-B; NFI-RED; NFIB; NFIB_HUMAN; NFIB3; Nuclear factor 1 B-type; Nuclear factor 1/B; Nuclear Factor 1B; Nuclear Factor I B; nuclear factor I/B; TGGCA Binding Protein; TGGCA-binding protein; NFIB2; NFIB3; HMGIC/NFIB.
literatures	Specific References  (1)     |     SL11899R has been referenced in 1 publications. [IF=4.36] Hanyue Ying. et al. CircATL2 enhances paclitaxel resistance of ovarian cancer via impacting miR-506-3p/NFIB axis. 2021 Sep 19  WB ;  human.   PubMed:34541682
Research Area	Cell biology  Neurobiology  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Chicken, Pig, Cow, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	47kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human NFIB/NF1B2: 1-100/420
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Function: Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Subunit: Binds DNA as a homodimer. Subcellular Location: Nucleus. Similarity: Belongs to the CTF/NF-I family. Contains 1 CTF/NF-I DNA-binding domain. SWISS: O00712 Gene ID: 4781 Database links: Entrez Gene: 4781 Human Entrez Gene: 18028 Mouse Entrez Gene: 29227 Rat Entrez Gene: 396209 Chicken Omim: 600728 Human SwissProt: P17924 Chicken SwissProt: P13622 Hamster SwissProt: O00712 Human SwissProt: P97863 Mouse Unigene: 644095 Human Unigene: 317947 Mouse
Product Picture	Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-NFIB (SL11899R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 48 kD Sample:293T Cell (Human) Lysate at 40 ug Primary: Anti-NFIB (SL11899R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 47 kD Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug Lane 2: A431 (Human) Cell Lysate at 30 ug Lane 3: Lung (Mouse) Lysate at 40 ug Lane 4: Ovary (Mouse) Lysate at 40 ug Lane 5: Kidney (Mouse) Lysate at 40 ug Primary: Anti-NFIB (SL11899R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 47 kD Blank control: A431. Primary Antibody (green line): Rabbit Anti-NFIB antibody (SL11899R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Totalgoods,subtotals:￥Checkout