Product Name	NFIA
Chinese Name	核因子1A抗体
Alias	CCAAT binding transcription factor; CCAAT Box Binding Transcription Factor; CCAAT-box-binding transcription factor; CTF; FLJ39164; NF I/A; NF-I/A; NF1 A; NF1-A; NF1A; NFI A; NFI L; NFI-A; NFIA; NFIA_HUMAN; Nuclear Factor 1 A; Nuclear factor 1 A type; Nuclear factor 1 A-type; Nuclear factor 1/A; Nuclear Factor 1A; Nuclear Factor I A; Nuclear factor I/A; nuclear factor I/X (CCAAT-binding transcription factor); ynuclear factor I/X (CCAAT-binding transcription factor); Nuclear factor I/X; Nuclear Factor IA; TGGCA binding protein; TGGCA-binding protein.
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, Chicken, Dog, Pig, Cow, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	56kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human NFIA/NF-1: 41-140/509
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] Function: Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Subunit: Binds DNA as a homodimer. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the CTF/NF-I family. Contains 1 CTF/NF-I DNA-binding domain. SWISS: Q12857 Gene ID: 4774 Database links: Entrez Gene: 4774 Human Entrez Gene: 18027 Mouse Entrez Gene: 25492 Rat Omim: 600727 Human SwissProt: Q12857 Human SwissProt: Q02780 Mouse SwissProt: P09414 Rat Unigene: 710546 Human Unigene: 740757 Human Unigene: 31274 Mouse Unigene: 409646 Mouse Unigene: 10550 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NFIA) Polyclonal Antibody, Unconjugated (SL11898R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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