Product Name	NELL2
Chinese Name	蛋白激酶CBinding proteinNELL2抗体
Alias	NRP2; A330108N19Rik; Mel91; MEL91 protein; NEL like 2 (chicken); NEL-like protein 2; Nel-related protein 2; NELL2; NELL2_HUMAN; NRP2; Protein kinase C-binding protein NELL2; R75516.
Research Area	Cell biology  Neurobiology  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Dog, Pig, Cow, Horse, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	89kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human NELL2/NRP2: 201-300/816
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a glycoprotein containing several von Willebrand factor C domains and epidermal growth factor (EGF)-like domains. The encoded protein acts as a homotrimer and is found in the cytoplasm. Several variants encoding a few different isoforms exist, and at least one isoform appears to be a secreted protein. Studies in mouse suggest that this protein plays a role in neural cell growth and differentiation as well as in oncogenesis. [provided by RefSeq, Feb 2009] Subunit: Homotrimer. Binds to PKC beta-1 Subcellular Location: Secreted. Similarity: Contains 6 EGF-like domains. Contains 1 TSP N-terminal (TSPN) domain. Contains 5 VWFC domains. SWISS: Q99435 Gene ID: 4753 Database links: Entrez Gene: 4753 Human Entrez Gene: 54003 Mouse Omim: 602320 Human SwissProt: Q99435 Human SwissProt: Q61220 Mouse Unigene: 505326 Human Unigene: 3959 Mouse
Product Picture	Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Mouse Placenta tissue lysates Lane 3: Rat Testis tissue lysates Lane 4: Rat Placenta tissue lysates Primary: Anti-NELL2 (SL11895R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 89 kDa Observed band size: 135 kDa Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NELL2) Polyclonal Antibody, Unconjugated (SL11895R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NELL2) Polyclonal Antibody, Unconjugated (SL11895R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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