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Product Name FRA2 Chinese Name fos相关抗原2抗体 Alias FLJ23306; Fos L2; FOS like antigen 2; Fos related antigen 2; FosL 2; FOSL2; FOSL2_HUMAN; FRA 2; fos-related antigen 2. Research Area Tumour Signal transduction Growth factors and hormones Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=3ug/test IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 36kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human FRA2: 231-326/326 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Fos and Jun dimerize to form Activator Protein 1 (AP1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13 acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the 'leucine-zipper' motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP1 dependent genes.
Function:
Controls osteoclast survival and size (By similarity). As a dimer with JUN, activates LIF transcription (By similarity). Activates CEBPB transcription in PGE2-activated osteoblasts (By similarity).
Subunit:
Heterodimer
Subcellular Location:
Nuclear
Similarity:
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP (basic-leucine zipper) domain.
SWISS:
P15408
Gene ID:
2355
Database links:Entrez Gene: 2355 Human
Entrez Gene: 421416 Chicken
Entrez Gene: 14284 Mouse
Omim: 601575 Human
SwissProt: P18625 Chicken
SwissProt: P15408 Human
SwissProt: P47930 Mouse
Unigene: 220971 Human
Unigene: 596972 Human
Unigene: 24684 Mouse
Unigene: 10962 Rat
Unigene: 163577 Rat
FOSL2蛋白对cell factor、生长因子、感染或致癌刺激等生理或病理信号发生应答,调节基因的转录, 参与细胞的增殖、分化等过程. FOSL2在Tumour形成及发展过程中, 通过促进细胞增殖、抑制分化、促进Tumour细胞的侵袭和转移等过程发挥作用.Product Picture Sample:
Lane 1: Mouse Small intestine tissue lysates
Lane 2: Rat Small intestine tissue lysates
Primary: Anti-FRA2 (SL1188R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 44 kD
Tissue/cell: mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FRA2/FOSL2 Polyclonal Antibody, Unconjugated(SL1188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FRA2/FOSL2 Polyclonal Antibody, Unconjugated(SL1188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: A549.
Primary Antibody (green line): Rabbit Anti-FRA2 antibody (SL1188R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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