Product Name	GPM6B
Chinese Name	神经The cell membraneglycoproteinM6b
Alias	M6b; Neuronal membrane glycoprotein M6b; rhombex 29; GPM6B_HUMAN.
Research Area	Cell biology  Developmental biology  Neurobiology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Pig, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	29kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GPM6B: 1-100/265
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	GPM6B belongs to the myelin proteolipid protein family and is implicated in neural development. It is expressed in neurons and glia; cerebellar Bergmann glia, in glia within white matter tracts of the cerebellum and cerebrum, and in embryonic dorsal root ganglia. Function: May be involved in neural development. Involved in regulation of osteoblast function and bone formation. Involved in matrix vesicle release by osteoblasts; this function seems to involve maintenance of the actin cytoskeleton. May be involved in cellular trafficking of SERT and thereby in regulation of serotonin uptake. Subunit: Interacts with SERT. Subcellular Location: Cell membrane; Multi-pass membrane protein (By similarity). Note=Colocalizes with SERT at the plasma membrane Tissue Specificity: Neurons and glia; cerebellar Bergmann glia, in glia within white matter tracts of the cerebellum and cerebrum, and in embryonic dorsal root ganglia. Similarity: Belongs to the myelin proteolipid protein family. SWISS: Q13491 Gene ID: 2824 Database links: Entrez Gene: 2824 Human Entrez Gene: 14758 Mouse Entrez Gene: 192179 Rat Omim: 300051 Human SwissProt: Q13491 Human SwissProt: P35803 Mouse SwissProt: Q9JJK1 Rat Unigene: 495710 Human Unigene: 222320 Mouse Unigene: 153513 Rat
Product Picture	Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-GPM6B (SL11847R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 36 kD Sample: U251(Human) Cell Lysate at 30 ug Primary: Anti-GPM6B  (SL11847R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 34 kD Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 0.2μg in 100 μL1X PBS containing 0.5% BSA(green).

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