Product Name	DNTNP
Chinese Name	背神经管核蛋白抗体
Alias	DNTNP; Dorsal neural-tube nuclear protein; FA53A_HUMAN; FAM53A; family with sequence similarity 53, member A; Protein FAM53A.
Research Area	Cell biology  Developmental biology  Neurobiology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Dog,  (predicted: Chicken, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	43kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human DNTNP/FAM53A: 255-340/398
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Dorsal neural-tube nuclear protein is a 398 amino acid nuclear protein. DNTNP is thought to play an important role in neural development by specifying dorsal cell fates within the neural tube. Widely expressed in the dorsal neural tube, DNTNP is most highly expressed in the dorsal regions of the midbrain, the hindbrain, the diencephalon, and the spinal neural tube and is expressed at lower levels in the branchial arches, the telencephalon, the heart, and somites of developing embryos. DNTNP is encoded by a gene located on chromosome 4 which contains many genes including the Huntingtin gene, which is found to encode an expanded glutamine tract in cases of Huntington's disease. Function: May play an important role in neural development; the dorsomedial roof of the third ventricle. Subcellular Location: Nucleus. Subnuclear distribution. Similarity: Belongs to the FAM53 family. SWISS: Q6NSI3 Gene ID: 152877 Database links: Entrez Gene: 152877 Human SwissProt: Q6NSI3 Human Unigene: 143314 Human
Product Picture	Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DNTNP) Polyclonal Antibody, Unconjugated (SL11839R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (dog testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DNTNP) Polyclonal Antibody, Unconjugated (SL11839R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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