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Product Name Emx1 Chinese Name 有脊椎动物脑发育相关蛋白Emx1抗体 Alias Empty spiracles homeobox 1; Empty spiracles homolog 1; empty spiracles homolog 1 Drosophila antibodyEmpty spiracles like protein 1; Empty spiracles-like protein 1; EMX1 ; EMX1_HUMAN ; Homeobox protein EMX1. Research Area Cell biology Developmental biology Neurobiology Signal transduction Stem cells transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 29kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Emx1: 201-257/257 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Emx1 and Emx2 are human homologs to the Drosophila developmental genes empty spiracles expressed in anterior body regions during early Drosophila embryogenesis. Emx1 and Emx2 are homeobox proteins expressed in the developing vertebrate brain. Emx2 is expressed in the dorsal telencephalon and small diencephalic regions, while Emx1 expression is exclusively confined to pyramidal neurons of the dorsal telencephalon. In the embryonic brain, Emx1 is expressed in both proliferating and differentiating neurons while Emx2 is expressed only in proliferating neurons. OTX1 and OTX2 are human homologs of the Drosophila developmental genes orthodenticle. In development, the sequence of expression begins with OTX2 at day 10 post coitum followed by OTX1, Emx2 and finally Emx1. The genes encoding human Emx1 and Emx2 map to chromosomes 2p14-p13 and 10q26.1, respectively.
Function:
Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.
Subcellular Location:
Nucleus.
Tissue Specificity:
Cerebral cortex.
Similarity:
Belongs to the EMX homeobox family.
Contains 1 homeobox DNA-binding domain.
SWISS:
Q04741
Gene ID:
2016
Database links:Entrez Gene: 2016 Human
Entrez Gene: 13796 Mouse
Omim: 600034 Human
SwissProt: Q04741 Human
SwissProt: Q04742 Mouse
Unigene: 516090 Human
Unigene: 391473 Mouse
Unigene: 140907 Rat
Product Picture Sample:
HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti- Emx1 (SL11838R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29kD
Observed band size: 29kD
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-Emx1 antibody (SL11838R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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