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Product Name Cux2 Chinese Name 转录同源蛋白质CUX2抗体 Alias CDP2; Cut like homeobox 2; Cut-like 2; CUTL2; CUX2; Homeobox protein cut-like 2; Homeobox protein cux 2; Homeobox protein Cux-2; KIAA0293; CUX2_HUMAN. literatures Specific References (1) | SL11832R has been referenced in 1 publications.[IF=2.74] Spinelli M et al. Synthetic PreImplantation Factor (sPIF) reduces inflammation and prevents preterm birth. PLoS One . 2020 Jun 8;15(6):e0232493. IHC-P ; Mouse.Research Area Neurobiology Signal transduction Stem cells transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 162kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Cux2: 1001-1085/1486 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Cux2 is a marker for cortical subpopulations. Cux2 is an orthologue of the Drosophila cut gene, which encodes a homeodomain protein involved in neuronal specification. During cortical development Cux2 identifies two subpopulations with different spatial origins, migratory behaviours and phenotypic characteristics: (i) a population of interneurons, which invades the pallium from the subpallium; and (ii) a neuronal population produced in the pallium around embryonic day 11.5, which divides in the SVZ and accumulates in the intermediate zone (IZ). Subsequently, Cux2 is a marker of upper cortical layers.
Function:
May be a transcription factor involved in neural specification. Binds to DNA in a sequence-specific manner
Subcellular Location:
Nuclear
Similarity:
Belongs to the CUT homeobox family.
Contains 3 CUT DNA-binding domains.
Contains 1 homeobox DNA-binding domain.
SWISS:
O14529
Gene ID:
23316
Database links:Entrez Gene: 23316 Human
Entrez Gene: 13048 Mouse
Omim: 610648 Human
SwissProt: O14529 Human
SwissProt: P70298 Mouse
Unigene: 124953 Human
Unigene: 156172 Mouse
Unigene: 332661 Mouse
Product Picture Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cux2 Polyclonal Antibody, Unconjugated(SL11832R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (human thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cux2) Polyclonal Antibody, Unconjugated (SL11832R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Cux2 Antibody(SL11832R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Cux2 Antibody(SL11832R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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