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Product Name phospho-LRRK2 (Thr1410) Chinese Name 磷酸化富亮氨酸重复激酶2抗体 Alias LRRK2 (phospho T1410); LRRK2 (phospho Thr1410); p-LRRK2 (Thr1410); Dardarin; Leucine rich repeat kinase 2; LRRK 2; LRRK-2; PARK 8; PARK8; ROCO 2; ROCO-2; ROCO2; Leucine-rich repeat serine/threonine-protein kinase 2; Dardarin; LRRK2_HUMAN; augmented in rheumatoid arthritis 17; AURA17; Leucine rich repeat kinase 2; RIPK7. Product Type Phosphorylated anti Research Area Cell biology Neurobiology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, Rabbit, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 286kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human LRRK2 around the phosphorylation site of Thr1410: FM(p-T)QR Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail LRRK2 (Dardarin) is encoded by the gene LRRK2. Genetic mutations of LRRK2 have been linked to Parkinsonism and synucleinopathies. LRRK2 belongs to the ROCO protein family and includes a protein kinase domain of the MAPKKK class and several other major functional domains.
Function:
May play a role in the phosphorylation of proteins central to Parkinson disease. Phosphorylates PRDX3. May also have GTPase activity.
Subunit:
Interacts with PARK2. Interacts with PRDX3. Interacts with TPCN2.
Subcellular Location:
Cytoplasm. Membrane. Mitochondrion. Localized in the cytoplasm and associated with cellular membrane structures. Associates with the mitochondrial outer membrane.
Tissue Specificity:
Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas.
DISEASE:
Defects in LRRK2 are the cause of Parkinson disease type 8 (PARK8) [MIM:607060]. A slowly progressive neurodegenerative disorder characterized by bradykinesia, rigidity, resting tremor, postural instability, neuronal loss in the substantia nigra, and the presence of neurofibrillary MAPT (tau)-positive and Lewy bodies in some patients.
Similarity:
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 12 LRR (leucine-rich) repeats.
Contains 1 protein kinase domain.
Contains 1 Roc domain.
SWISS:
Q5S007
Gene ID:
120892
Database links:Entrez Gene: 120892 Human
Entrez Gene: 66725 Mouse
Omim: 609007 Human
SwissProt: Q5S007 Human
SwissProt: Q5S006 Mouse
Unigene: 187636 Human
Unigene: 37558 Mouse
Unigene: 213971 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-LRRK2 (Thr1410)) Polyclonal Antibody, Unconjugated (SL11786R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-LRRK2 (Thr1410)) Polyclonal Antibody, Unconjugated (SL11786R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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