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Product Name TMEM158 Chinese Name Transmembrane protein158抗体 Alias 40 kDa BINP binding protein; 40 kDa BINP-binding protein; BBP; BINP receptor; Brain injury derived neurotrophic peptide (BINP) binding protein; Brain specific binding protein; DKFZp586E1621; HBBP; p40BBP; Ras induced senescence protein 1; Ras-induced senescence protein 1; RIS1; TM158_HUMAN; TMEM 158; TMEM158; Transmembrane protein 158. Research Area Tumour Cell biology Neurobiology Apoptosis Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Chicken, Pig, Cow, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 28kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human TMEM158: 188-260/300 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail TMEM158 is a 286 amino acid protein encoded by a gene mapping to human chromosome 3. Chromosome 3 is made up of about 214 million bases encoding over 1,100 genes. Notably, there is a chemokine receptor gene cluster and a variety of human cancer related loci on chromosome 3. Particular regions of the chromosome 3 short arm are deleted in many types of cancer cells. Key tumor suppressing genes on chromosome 3 encode apoptosis mediator RASSF1, cell migration regulator HYAL1 and angiogenesis suppressor SEMA3B. Marfan Syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth Disease are a few of the numerous genetic diseases associated with chromosome 3.
Function:
Receptor for brain injury-derived neurotrophic peptide (BINP), a synthetic 13-mer peptide.
Subcellular Location:
Membrane.
Post-translational modifications:
N-glycosylated.
Similarity:
Belongs to the TMEM158 family.
SWISS:
Q8WZ71
Gene ID:
25907
Database links:
Entrez Gene: 25907 Human
Entrez Gene: 72309 Mouse
SwissProt: Q8WZ71 Human
SwissProt: Q6F5E0 Mouse
Unigene: 740403 Human
Unigene: 8569 Mouse
Unigene: 4294 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEM158) Polyclonal Antibody, Unconjugated (SL11770R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TMEM158 Polyclonal Antibody, Unconjugated(SL11770R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEM158) Polyclonal Antibody, Unconjugated (SL11770R) at 1:400 overnight at 4°C, followed by a conjugated secondary (SL0295G-Cy3) at [1:500] for 90 minutes and DAPI staining of the nuclei.Blank control: Mouse spleen cells(blue).
Primary Antibody: Rabbit Anti-TMEM158 antibody(SL11770R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Blank control: Mouse heart cells(blue).
Primary Antibody: Rabbit Anti-TMEM158 antibody(SL11770R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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