Product Name	OPA1
Chinese Name	视神经萎缩相关蛋白1抗体
Alias	Dynamin like 120 kDa protein; Dynamin like 120 kDa protein, mitochondrial; Dynamin-like 120 kDa protein; Dynamin-like 120 kDa protein, form S1; FLJ12460; Juvenile kjer type optic atrophy; Juvenile kjer-type optic atrophy; KIAA0567; KJER type; Large GTP binding protein; largeG; MGM1; Mitochondrial dynamin like 120 kDa protein; Mitochondrial dynamin like GTPase; NPG; NTG; OAK; OPA 1; OPA1; OPA1 gene; OPA1_HUMAN; Optic atrophy 1 (autosomal dominant); OPTIC ATROPHY 1; Optic atrophy 1 gene protein; Optic atrophy 1 homolog (human); Optic atrophy protein 1; Optic atrophy protein 1 homolog.
literatures	Specific References  (13)     |     SL11764R has been referenced in 13 publications. [IF=13.352] Guangdong Bai. et al. Perinatal exposure to glyphosate-based herbicides impairs progeny health and placental angiogenesis by disturbing mitochondrial function. ENVIRON INT. 2022 Dec;170:107579  WB ;  Pig.   PubMed:36265358 [IF=6.792] Yang Q et al. Hexavalent chromium induces mitochondrial dynamics disorder in rat liver by inhibiting AMPK/PGC-1α signaling pathway. Environ Pollut.2020 Oct;265(Pt A):114855.  WB ;  Rat.   PubMed:32474337 [IF=6.513] Zhe Li. et al. Bisphenol A aggravate selenium deficiency-induced apoptosis via miR-215-3p/Dio1 to activate ROS/PI3K/AKT pathway in chicken arterial. J CELL PHYSIOL. 2023 Apr;:  WB ;  Chicken.   PubMed:37012668 [IF=5.895] Xixi Wang. et al. Cannabidiol Alleviates Perfluorooctanesulfonic Acid-Induced Cardiomyocyte Apoptosis by Maintaining Mitochondrial Dynamic Balance and Energy Metabolic Homeostasis. J AGR FOOD CHEM. 2023;XXXX(XXX):XXX-XXX  WB ;  Mouse,Rat.   PubMed:37010249 [IF=5.396] Yi Zhao. et al. Role of mitochondria-endoplasmic reticulum coupling in lycopene preventing DEHP-induced hepatotoxicity. Food Funct. 2021 Sep;:  WB ;  mouse.   PubMed:34608470 [IF=4.527] Wang S et al. Hydrogen sulfide-induced oxidative stress leads to excessive mitochondrial fission to activate apoptosis in broiler myocardia. Ecotoxicol Environ Saf. 2019 Aug 20;183:109578.  WB ;  broiler.   PubMed:31442807 [IF=4.427] Wang Y et al.Fine particulate matter induces mitochondrial dysfunction and oxidative stress in human SH-SY5Y cells.(2018) Chemosphere;218:577-588.  WB ;   PubMed:30502696 [IF=3.708] Xuena Zhang. et al. Luteoloside Prevents Sevoflurane-induced Cognitive Dysfunction in Aged Rats via Maintaining Mitochondrial Function and Dynamics in Hippocampal Neurons. NEUROSCIENCE. 2023 Feb;:  WB ;  Rat.   PubMed:36764603 [IF=3.7] Ku, Tingting, et al. "PM 2.5, SO 2 and NO 2 co-exposure impairs neurobehavior and induces mitochondrial injuries in the mouse brain."Chemosphere 163 (2016): 27-34.  WB ;  Mouse.   PubMed:27521637 [IF=2.835] Yuan Zhang a et al. Electroacupuncture ameliorates endotoxin-induced acute lung injury in rabbits by regulating heme oxygenase-1 expression to improve mitochondrial dynamics. Journal of King Saud University – Science, 2019.  WB ;  Rabbit.   PubMed:DOI:10.1016/j.jksus.2019.11.029 [IF=2.752] Yuliang Wen. et al. Changes in the Mitochondrial Dynamics and Functions Together with the mRNA/miRNA Network in the Heart Tissue Contribute to Hypoxia Adaptation in Tibetan Sheep. Animals-Basel. 2022 Jan;12(5):583  IHC ;  Sheep.   PubMed:35268153 [IF=2.323] Guan Wang. et al. Effects of Aging on Expression of Mic60 and OPA1 and Mitochondrial Morphology in Myocardium of Tibetan Sheep. Animals-Basel. 2020 Nov;10(11):2160  IHC ;  Sheep.   PubMed:33233488 [IF=1.35] Hui, Yan, et al. "Resveratrol improves mitochondrial function in the remnant kidney from 5/6 nephrectomized rats." Acta Histochemica (2017).  WB ;  Rat.   PubMed:28434671
Research Area	Cardiovascular  Cell biology  Neurobiology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	111kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human OPA1: 651-750/960
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	OPA1 is a 120kDa protein belonging to the dynamin family. The OPA1 gene has been localized to 3q29. The gene is targeted to mitochondria and is involved in mitochondrial biogenesis. Defects in OPA1 are a cause of optic atrophy type 1. OPA1 is mostly expressed in retina but can also be expressed in brain, testis, heart and skeletal muscle. Function: Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space. Subcellular Location: Mitochondrion inner membrane. Mitochondrion intermembrane space. Tissue Specificity: Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues. Post-translational modifications: PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion. DISEASE: Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) [MIM:165500]. OPA1 is a dominantly inherited optic neuropathy occurring in 1 in 50,000 individuals that features progressive loss in visual acuity leading, in many cases, to legal blindness. Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy. Similarity: Belongs to the dynamin family. SWISS: O60313 Gene ID: 4976 Database links: Entrez Gene: 424900 Chicken Entrez Gene: 4976 Human Entrez Gene: 74143 Mouse Entrez Gene: 171116 Rat Omim: 605290 Human SwissProt: O60313 Human SwissProt: P58281 Mouse SwissProt: Q2TA68 Rat Unigene: 594504 Human Unigene: 274285 Mouse Unigene: 9783 Rat
Product Picture	Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-OPA1 (SL11764R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 111 kD Observed band size: 111 kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-OPA1 Polyclonal Antibody, Unconjugated(SL11764R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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