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Product Name SULT4A1 Chinese Name 脑磺基转移酶样蛋白4A1抗体 Alias BR STL 1; Brain sulfotransferase like protein; Brain sulphotransferase like; BRSTL1; hBR STL 1; hBR STL; Nervous system cytosolic sulfotransferase; Nervous system sulfotransferase; NST; Sulfotransferase 4A1; Sulfotransferase family 4A member 1; Sulfotransferase related protein; SULTX3; ST4A1_HUMAN. Research Area Tumour Neurobiology Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 33kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SULT4A1: 221-284/284 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Sulfation is an essential conjugation reaction that increases the water solubility of many compounds, thereby influencing their renal excretion and also resulting in the formation of active metabolites. SULT4A1 (sulfotransferase family 4A, member 1), whose alternative names include brain sulfotransferase-like protein, nervous system sulfotransferase, NST, SULTX3, hBR-STL-1, BRSTL1, BR-STL-1, MGC40032 and DJ388M5.3, is a 284 amino acid protein showing cytoplasmic localization. As a member of the sulfotransferase 1 family, SULT4A1 plays a role in elimination of xenobiotics, activation of procarcinogens and regulation of hormones. SULT4A1 is highly expressed in cerebral cortex and frontal lobe, with lower expression in cerebellum, temporal and occipital lobes. Two SULT4A1 isoforms exist to alternative splicing events. The gene encoding SULT4A1 maps to human chromosome 22q13.2, a region which has been implicated in predisposition to schizophrenia.
Function:
SULT4A1 is a member of the sulfotransferase family. It is a brain-specific sulfotransferase believed to be involved in the metabolism of neurotransmitters. It may catalyze the sulfate conjugation of many drugs, xenobiotic compounds, hormones, and neurotransmitters. Polymorphisms in this gene may be associated with susceptibility to schizophrenia. There are two named isoforms.
Subcellular Location:
Cytoplasmic
Tissue Specificity:
Highly expressed in the cerebral cortex andfrontal lobe, slightly less in the cerebellum, occipital andtemporal lobes, relatively low in the medulla and putamen, andlowest in the spinal cord. No expression detected in the pancreas(PubMed:10698717). Highly expressed in fetal brain and occipitallobe, slightly less in the whole brain, frontal lobe, hippocampus,and lung, very low expression in cerebellum, medulla oblongata,temporal lobe, testis, kidney and appendix (PubMed:12039030).
Similarity:
Belongs to the sulfotransferase 1 family.
SWISS:
Q9BR01
Gene ID:
25830
Database links:
Entrez Gene: 25830 Human
Entrez Gene: 29859 Mouse
Entrez Gene: 100009444 Rabbit
Omim: 608359 Human
SwissProt: Q9BR01 Human
SwissProt: P63046 Mouse
Unigene: 189810 Human
Unigene: 248796 Mouse
Unigene: 162965 Rat
Product Picture Positive sample:Paraformaldehyde-fixed, paraffin embedded (rat brain); Negative sample:Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SULT4A1) Polyclonal Antibody, Unconjugated (SL11763R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SULT4A1 Polyclonal Antibody, Unconjugated(SL11763R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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