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Product Name SCGN Chinese Name 促泌素抗体 Alias SEGN_HUMAN; Secretagogin; SECRET; secretagogin, EF-hand calcium binding protein; SEGN; CALBL; setagin; DJ501N12.8; literatures Specific References (2) | SL11754R has been referenced in 2 publications.[IF=5.542] Radhika Khandelwal. et al. Extracellular secretagogin is internalized into the cells through endocytosis. 2022 Jan 09 ICC ; Mouse.[IF=5.486] Amrutha H. Chidananda. et al. Secretagogin is a Ca2+-dependent stress-responsive chaperone that may also play a role in aggregation-based proteinopathies. J BIOL CHEM. 2022 Jul;:102285 WB ; Rat.Research Area Cell biology Neurobiology Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Horse, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 32kDa Cellular localization cytoplasmic The cell membrane Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Secretagogin: 221-276/276 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq, Jul 2008]
Subcellular Location:
Cytoplasm. Secreted.Cytoplasmic vesicle, secretory vesicle membrane; Peripheralmembrane protein; Cytoplasmic side.Note=Predominantly cytoplasmic. A small proportion is associatedwith secretory granules and membrane fractions.Detectable in human serum after ischemic neuronal damage.
Tissue Specificity:
Expressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells. In the brain, the expression is restricted to distinct subtypes of neurons with highest expression in the molecular layer of the cerebellum (stellate and basket cells), in the anterior part of the pituitary gland, in the thalamus, in the hypothalamus and in a subgroup of neocortical neurons.
Similarity:
Contains 6 EF-hand domains.
SWISS:
O76038
Gene ID:
10590
Database links:Entrez Gene: 10590 Human
SwissProt: O76038 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCGN) Polyclonal Antibody, Unconjugated (SL11754R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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