Product Name	ASCL1
Chinese Name	神经母细胞特异性转移因子抗体
Alias	Achaete scute complex homolog 1; MASH1; MASH1/Achaete-scute homolog 1; Achaete scute complex homolog like 1; Achaete scute complex homologue 1; Achaete scute complex homologue like 1; Ascl 1; Ascl1; Ash 1; Ash1; Hash 1;Hash1; Mammalian achaete scute homolog 1; Mammalian achaete scute homologue 1; Mash 1; Achaete-scute homolog 1; ASCL1_HUMAN; ASH-1; hASH1; Class A basic helix-loop-helix protein 46; bHLHa46.
literatures	Specific References  (3)     |     SL1155R has been referenced in 3 publications. [IF=5.41] Misra et al. Asymmetric activation of Dll4-Notch signaling by Foxn4 and proneural factors activates BMP/TGFβ signaling to specify V2b interneurons in the spinal cord. (2014) Development. 141:187-98  ChIP ;  Mouse.   PubMed:24257627 [IF=2.886] Xiao Fu. et al. PD-L1 Predicts Poor Prognosis in Surgically Resected Limited Stage Small-Cell Lung Cancer. Cancer Manag Res. 2020; 12: 10939–10948  IHC ;  Human.   PubMed:33154673 [IF=2.535] Song X et al. Anti-aging effects exerted by Tetramethylpyrazine enhances self-renewal and neuronal differentiation of rat bMSCs by suppressing NF-kB signaling. Biosci Rep. 2019 Jun 25;39(6). pii: BSR20190761.  WB ;  Rat.   PubMed:31171713
Research Area	Neurobiology  Signal transduction  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Cow, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	26kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ASCL1: 151-236/236
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. [provided by RefSeq, Jul 2008] Function: Transcriptional regulator. May play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Involved in the initiation of neuronal differentiation. Mediates transcription activation by binding to the E box (5'-CANNTG-3'). Subunit: Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3. Subcellular Location: Nucleus (Probable). Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. SWISS: P50553 Gene ID: 429 Database links: Entrez Gene: 429 Human Entrez Gene: 17172 Mouse Entrez Gene: 64186 Rat Omim: 100790 Human SwissProt: P50553 Human SwissProt: Q02067 Mouse SwissProt: P19359 Rat Unigene: 703025 Human Unigene: 136217 Mouse Unigene: 32936 Rat ASCL1/ASH1是一种神经母细胞特异性转移因子,可以促进细胞向一定方向分化但却抑制了最终的分化环节,造成细胞停留于不成熟阶段.
Product Picture	Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-ASCL1 (SL1155R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 26 kD Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MASH1 Polyclonal Antibody, Unconjugated(SL1155R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: mouse embryos tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MASH1 Polyclonal Antibody, Unconjugated(SL1155R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MASH1 Polyclonal Antibody, Unconjugated(SL1155R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MASH1 Polyclonal Antibody, Unconjugated(SL1155R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (MASH1) polyclonal Antibody, Unconjugated (SL1155R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control:A549. Primary Antibody (green line): Rabbit Anti-MASH1 antibody (SL1155R) Dilution: 2ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control:Mouse brain. Primary Antibody (green line): Rabbit Anti-MASH1 antibody (SL1155R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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