Product Name	Centaurin alpha 1
Chinese Name	Centaurin α1抗体
Alias	ArfGAP with dual PH domains 1; CENTA 1; CENTA1; Centaurin alpha; GCS1L; IP4/PIP3 binding protein; p42IP4; Putative MAPK activating protein PM25; ADAP1_HUMAN.
Research Area	Cell biology  Neurobiology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, Dog, Pig, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	43kDa
Cellular localization	The nucleus cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Centaurin alpha 1: 101-200/374
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	ADP-ribosylation factor (ARF) family of small GTP-binding proteins are involved in vesicular transport regulation and in controlling cytoskeletal organization and cell adhesion. These proteins are best characterized as regulators of membrane traffic. The Centaurin GTPase-activating protein family comprise a subset of ARF regulatory molecules that transduce PI 3-kinase activation into coordinated control of Arf-dependent pathways. This family includes ASAP1, ACAP1, ACAP2, AGAP1, ARAP1, ARAP2, Centaurin a1 and Centaurin g3. The Centaurin a1 protein is a high affinity PtdIns(3,4,5)P3 binding protein enriched in brain. By acting as a GTPase activating protein for ADP ribosylation factor 6 (ARF6), Centaurin a1 is able to switch off ARF6 and inhibit its ability to mediate b2-adrenoceptor internalization and negatively regulate ARF6 activity by functioning as an in vivo PIP3 dependent ARF6 GAP. Function: The centaurin alpha 1 protein is a high affinity PtdIns(3,4,5)P3 binding protein enriched in brain. By acting as a GTPase activating protein for ADP ribosylation factor 6 (ARF6), centaurin alpha 1 is able to switch off ARF6 and inhibit its ability to mediate beta 2 adrenoceptor internalization and negatively regulates ARF6 activity by functioning as an in vivo PIP3 dependent ARF6 GAP. Subunit: Interacts with PRKCA, PRKCI and PRKCZ. Interacts with the N-terminal region of PRKD1. Subcellular Location: Cellular localizationCytoplasmic, Nuclear and Plasma membrane Tissue Specificity: Expressed at highest levels in brain and at lower levels in peripheral blood leukocytes. Post-translational modifications: Phosphorylated by PRKCA, PRKCI, PRKCZ and PRKD1 in vitro. Similarity: Contains 1 Arf-GAP domain. Contains 2 PH domains. SWISS: O75689 Gene ID: 11033 Database links: Entrez Gene: 11033 Human Entrez Gene: 231821 Mouse Entrez Gene: 397453 Pig Entrez Gene: 171097 Rat Omim: 608114 Human SwissProt: O75689 Human Unigene: 297819 Mouse Unigene: 10539 Rat
Product Picture	Tissue/cell: Rat brain ; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Centaurin alpha 1 Polyclonal Antibody, Unconjugated(SL11543R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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