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Product Name EXOC3 Chinese Name 胞吐分泌蛋白Sec6抗体 Alias EXOC 3; Exocyst complex component 3; Exocyst complex component Sec 6; Exocyst complex component Sec6; rSec 6; rSec6; Sec 6; Sec-6; Sec 6 homolog; Sec 6p; Sec6; SEC6 L1; SEC6 like 1; Sec6 protein; SEC6L1; Sec6p; EXOC3_HUMAN. Research Area Cell biology Neurobiology Cell type markers Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Dog, Cow, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 87kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human EXOC3: 714-756/756 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity.
Function:
Sec6 is a component of the exocyst complex involved in the docking of exocystic vesicles with fusions site on the plasma membrane.
Subunit:
The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Interacts with EXOC3L1 (By similarity). Interacts with BIRC6/bruce. Interacts with MYRIP
Subcellular Location:
Cytoplasmic
Similarity:
Belongs to the SEC6 family.
SWISS:
O60645
Gene ID:
11336
Database links:Entrez Gene: 11336 Human
Entrez Gene: 211446 Mouse
Omim: 608186 Human
SwissProt: O60645 Human
SwissProt: Q6KAR6 Mouse
Unigene: 481464 Human
Unigene: 489612 Mouse
Unigene: 6753 Rat
Product Picture Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-rSec6 antibody (SL11396R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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