TEL: +86 571 56623320 EMAIL: [email protected]
Product Name SNAPIN Chinese Name 突触相关蛋白25Binding protein抗体 Alias SNAP associated protein; SNAP-25-binding protein; SNAP-associated protein; SNAP25BP; SNAPAP; SNAPIN; SNAPN_HUMAN; SNARE associated protein snapin; SNARE-associated protein Snapin; Synaptosomal associated protein 25 binding protein; Synaptosomal-associated protein 25-binding protein; OTTHUMP00000035157. Research Area Cell biology Neurobiology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 15kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SNAP25BP: 51-136/136 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a coiled-coil-forming protein that associates with the SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) complex of proteins and the BLOC-1 (biogenesis of lysosome-related organelles) complex. Biochemical studies have identified additional binding partners. As part of the SNARE complex, it is required for vesicle docking and fusion and regulates neurotransmitter release. The BLOC-1 complex is required for the biogenesis of specialized organelles such as melanosomes and platelet dense granules. Mutations in gene products that form the BLOC-1 complex have been identified in mouse strains that are models of Hermansky-Pudlak syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012].
Function:
May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release by potentiating the interaction of synaptotagmins with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells.
Subunit:
Interacts with CSNK1D, SNAP23 and STX4A but not with STX1A, VAMP2 and SYT1. Interacts with SNAP25; the interaction with SNAP25 is increased by its phosphorylation (By similarity). Component of the biogenesis of lysosome-related organelles complex 1 (BLOC-1) composed of BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. Octamer composed of one copy each BLOC1S1, BLOC1S2, BLOC1S3, BLOC1S4, BLOC1S5, BLOC1S6, DTNBP1/BLOC1S7 and SNAPIN/BLOC1S8. The BLOC-1 complex associates with the AP-3 protein complex and membrane protein cargos. Associates with the SNARE complex. Interacts with CEP110, NANOS1, PUM2 and RGS7. Interacts with human cytomegalovirus/HHV-5 protein UL70.
Subcellular Location:
Cytoplasm (By similarity). Membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Cell junction, synapse, synaptosome. Cytoplasm, perinuclear region. Golgi apparatus membrane (By similarity). Note=May be cytoplasmic and peripheral membrane bound or anchored to the vesicular membrane through an N-terminal signal anchor (By similarity). Co-localizes with NANOS1 and PUM2 in the perinuclear region of germ cells.
Tissue Specificity:
Expressed in male germ cells of adult testis (at protein level).
Post-translational modifications:
Phosphorylated by CSNK1D/CK1 (By similarity).
Similarity:
Belongs to the SNAPIN family.
SWISS:
O95295
Gene ID:
23557
Database links:Entrez Gene: 23557 Human
Entrez Gene: 20615 Mouse
Omim: 607007 Human
SwissProt: O95295 Human
SwissProt: Q9Z266 Mouse
Unigene: 32018 Human
Unigene: 331182 Mouse
Unigene: 3815 Rat
Scan Wechat Qrcode