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Product Name C1QA Chinese Name 补体C1qα链多肽抗体 Alias Complement C1q subcomponent subunit A; C1Q; C1QA_HUMAN; Complement C1q subcomponent subunit A; C1QA; C1QA_HUMAN; literatures Specific References (2) | SL11336R has been referenced in 2 publications.[IF=7.109] Wu Yutong. et al. Reduced osteoclast-derived apoptotic bodies in bone marrow characterizes the pathological progression of osteoporosis. CELL DEATH DISCOV. 2023 Apr;9(1):1-9 WB ; Mouse.[IF=5.156] Liu, Tao. et al. Microarray-based analysis of renal complement components reveals a therapeutic target for lupus nephritis. Arthritis Res Ther. 2021 Dec;23(1):1-15 IHC ; Human.Research Area immunology Neurobiology Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, ) Applications WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 24kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human C1QA: 61-160/245 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes the A-chain polypeptide of serum complement subcomponent C1q, which associates with C1r and C1s to yield the first component of the serum complement system. C1q deficiency is associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains which include 6 A-chains, 6 B-chains, and 6 C-chains. Each chain contains an N-terminal collagen-like region and a C-terminal C1q globular domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]
Function:
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
Subunit:
C1 is a calcium-dependent trimolecular complex of C1q, R and S in the molar ration of 1:2:2. C1q subcomponent is composed of nine subunits, six of which are disulfide-linked dimers of the A and B chains, and three of which are disulfide-linked dimers of the C chain.
Subcellular Location:
Secreted.
Post-translational modifications:
O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
DISEASE:
Defects in C1QA are the cause of complement component C1qA deficiency (C1QAD) [MIM:120550]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
Similarity:
Contains 1 C1q domain.
Contains 1 collagen-like domain.
SWISS:
P02745
Gene ID:
712
Database links:Entrez Gene: 712 Human
Entrez Gene: 12259 Mouse
SwissProt: P02745 Human
SwissProt: P14106 Mouse
Product Picture Sample:
K562(Human) Cell Lysate at 30 ug
Primary: Anti-C1Q (SL11336R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 25 kD
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C1Q) Polyclonal Antibody, Unconjugated (SL11336R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-C1Q Polyclonal Antibody, Unconjugated(SL11336R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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