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Product Name AP180 Chinese Name 装配衔接蛋白180抗体 Alias AP 180; AP3; Assembly Protein 180 kDa; Assembly Protein AP180; CALM; Clathrin coat assembly protein; Clathrin coat assembly protein AP180; Clathrin coat associated protein AP180; DKFZp781O051; F120; KIAA0656; NP185; pp155; SNAP 91; SNAP91; 91kDa synaptosomal associated protein; Synaptosomal associated protein 91 kD homolog; Synaptosomal associated protein 91 kDa homolog; AP180_HUMAN. literatures Specific References (1) | SL11333R has been referenced in 1 publications.[IF=9.988] Yajie Guo. et al. Perfluorooctanoic acid-induced developmental cardiotoxicity in chicken embryo: Roles of miR-490-5p. ENVIRON POLLUT. 2022 Nov;312:120022 WB ; Chicken.Research Area Cell biology Neurobiology Cell type markers Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Chicken, Dog, Pig, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 92kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human AP180: 201-300/907 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Clathrin-coated pits and vesicles are assembled for receptor-mediated endocytosis through interaction with Clathrin associated protein complexes. Vesicle transport is mediated from the trans-Golgi network by the adapter complex AP-1 and from the plasma membrane by the AP-2 complex. The AP-1 and AP-2 adapter protein complexes consist of Clathrin binding Adaptin proteins (g and b1 for AP-1, a and b2 for AP-2) and two smaller subunits known as AP50 and AP17. The a and b Adaptin chains have a similar two-domain organization with C-terminal domains that vary in both sequence and length. a-Adaptin splice variants A and C display variable relative expression levels and differential distribution in different tissues. AP-3 (also designated AP180 or F1-20) is a synapse-specific Clathrin assembly protein. The protein CALM (Clathrin assembly protein lymphoid myeloid leukemia) is highly homologous to AP180 and may also be involved in Clathrin assembly.
Function:
AP180 is a clathrin binding phospho-protein and faciliates the formation of clathrin coats. This protein is also known as pp155, NP185, F1-20, and AP-3. During neurotransmitter release synaptic vesicles fuse with the presynaptic plasma membrane. A whole protein machinery consisting of amphiphysin, clathrin, endophilin and synaptojanin is involved in the subsequent endocytotic recycling of the synaptic vesicles.
Subunit:
Binds AP2A2. Interacts with AP2B1; clathrin competes with SNAP91 (By similarity).
Subcellular Location:
Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane.
Post-translational modifications:
Thr-310 can be modified by the addition of N-acetylglucosamine which can be further phosphorylated. There is no evidence for direct Thr-310 phosphorylation (By similarity).
Similarity:
Contains 1 ENTH (epsin N-terminal homology) domain.
SWISS:
O60641
Gene ID:
9892
Database links:Entrez Gene: 9892 Human
Entrez Gene: 20616 Mouse
Omim: 607923 Human
SwissProt: O60641 Human
SwissProt: Q61548 Mouse
Unigene: 368046 Human
Unigene: 281651 Mouse
Unigene: 11022 Rat
Product Picture Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AP180 Polyclonal Antibody, Unconjugated(SL11333R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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