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Product Name CLIP2 Chinese Name 细胞质连接蛋白2抗体 Alias CAP GLY domain containing linker protein 2; CAP-Gly domain-containing linker protein 2; CYLN2; CLIP 115; CLIP; CLIP-115; CLIP2; CLIP2_HUMAN; cytoplasmic linker 2; Cytoplasmic linker protein 115; Cytoplasmic linker protein 2; KIAA0291; MGC11333; restin; Similar to RESTIN (CYTOPLASMIC LINKER PROTEIN 170 ALPHA 2); WBSCR3; WBSCR4; Williams Beuren syndrome chromosome region 3; Williams Beuren syndrome chromosome region 4; Williams-Beuren syndrome chromosomal region 3 protein; Williams-Beuren syndrome chromosomal region 4 protein; WSCR3; WSCR4. Research Area Neurobiology Signal transduction Cell adhesion molecule Cytoskeleton Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 116kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CYLN2: 901-1046/1046 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene belongs to the family of cytoplasmic linker proteins, which have been proposed to mediate the interaction between specific membranous organelles and microtubules. This protein was found to associate with both microtubules and an organelle called the dendritic lamellar body. This gene is hemizygously deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]
Function:
Seems to link microtubules to dendritic lamellar body (DLB), a membranous organelle predominantly present in bulbous dendritic appendages of neurons linked by dendrodendritic gap junctions. May operates in the control of brain-specific organelle translocations.
Subunit:
Interacts with CLASP1 and CLASP2.
Subcellular Location:
Cytoplasm. Cytoplasm; cytoskeleton. Associated with the cytoskeleton.
DISEASE:
Note=CLIP2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of CLIP2 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
Similarity:
Contains 2 CAP-Gly domains.
SWISS:
Q9UDT6
Gene ID:
7461
Database links:Entrez Gene: 7461 Human
Entrez Gene: 269713 Mouse
Omim: 603432 Human
SwissProt: Q9UDT6 Human
SwissProt: Q9Z0H8 Mouse
Unigene: 647018 Human
Unigene: 255138 Mouse
Unigene: 10893 Rat
Product Picture Sample:
Large intestine (Mouse) Lysate at 40 ug
Primary: Anti-CLIP2 (SL11314R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 116 kD
Observed band size: 116 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CLIP2) Polyclonal Antibody, Unconjugated (SL11314R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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