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Product Name GRK6 Chinese Name G protein-coupled receptor激酶6抗体 Alias GRK 6; GRK-6; G protein coupled receptor kinase 6; G protein coupled receptor kinase GRK6; G protein-coupled receptor kinase 6; G protein-coupled receptor kinase GRK6; G-protein-coupled receptor kinase 6; Gprk6; Grk6; GRK6_HUMAN. Research Area Cardiovascular Signal transduction Kinases and Phosphatases G protein-coupled receptor G protein signal Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 66kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human GRK6: 181-280/576 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonist-mediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first class is comprised of the second messenger-regulated kinases, such as c-AMP dependent protein kinase A and protein kinase C. The second class includes the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase (GRK 1), two forms of Beta-adrenergic receptor kinase: GRK 2 (Beta ARK, Beta ARK1) and GRK 3 (Beta ARK2), IT-11 (GRK 4), GRK 5, GRK 6 and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.
Function:
Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamin receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro).
Subcellular Location:
Membrane; Lipid-anchor.
Tissue Specificity:
Widely expressed.
Post-translational modifications:
It is uncertain whether palmitoylation is on Cys-561 and/or Cys-562 and/or Cys-565.
Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 1 RGS domain.
SWISS:
P43250
Gene ID:
2870
Database links:Entrez Gene: 2870 Human
Entrez Gene: 26385 Mouse
Omim: 600869 Human
SwissProt: P43250 Human
SwissProt: O70293 Mouse
Unigene: 235116 Human
Unigene: 10193 Mouse
Unigene: 10633 Rat
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