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Product Name FLIP Chinese Name FLIP抗体 Alias CASP8 and FADD-like apoptosis regulator subunit p12; c FLIP; c FLIPL; c FLIPR; c FLIPS; CASH; CASP8 and FADD like regulator; CASP8 and FADD like apoptosis regulator precursor; CASP8AP1; Caspase Eight Related Protein; Caspase homolog; Caspase Homologue; Caspase Like Apoptosis Regulatory Protein; Caspase related inducer of apoptosis; CASPER; Cellular FLICE like inhibitory protein; CFLA; CFLAR; CLARP; FADD like anti apoptotic molecule; FADD Like Anti-apoptotic Molecule 1; FADD like antiapoptotic molecule 1; FADD Like Apoptosis Regulator; FLAME 1; FLAME; FLAME1; FLICE Inhibitor Protein; FLIP; FLIPs; I FLICE; Inhibitor of FLICE; MACH Related Inducer of Toxicity; mFLIP; MRIT; USURPIN; Usurpin beta; FLICE-like inhibitory protein long form. Research Area Cell biology Signal transduction Apoptosis Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 12/52kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CASP8 and FADD-like apoptosis regulator subunit p12 (Potential): 401-480/480 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011].
Function:
Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
Tissue Specificity:
Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
Post-translational modifications:
Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.
Similarity:
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains.
SWISS:
O15519
Gene ID:
8837
Database links:Entrez Gene: 8837 Human
Entrez Gene: 12633 Mouse
Omim: 603599 Human
SwissProt: O15519 Human
SwissProt: O35732 Mouse
Unigene: 390736 Human
Unigene: 336848 Mouse
FLIP参与凋亡的调节。此抗体在长型和短型的FLIP异构体中均表达。短型FLIP包含2个死亡效应基因结构区,同源于FAS相关蛋白死亡效应基因结构区。长型FLIP包含1个附加的Caspase 样结构区,但是他缺少一个催化部位和在大多数Caspase 蛋白中形成底物结合束的残基。Product Picture Sample:
Heart(Mouse) Cell Lysate at 40 ug
Primary: Anti-FLIP (SL1120R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/52 kD
Observed band size: 52 kD
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-FLIP (SL1120R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/52 kD
Observed band size: 52 kD
Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FLIP Polyclonal Antibody, Unconjugated(SL1120R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FLIP Polyclonal Antibody, Unconjugated(SL1120R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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