Product Name	LHX2
Chinese Name	调控胚胎Stem cells分化蛋白Lhx2抗体
Alias	hLhx2; Homeobox protein LH-2; Homeobox protein LH2; LH2; LHX2; LHX2_HUMAN; LIM homeobox 2; LIM homeobox protein 2; LIM HOX gene 2; LIM/homeobox protein Lhx2.
literatures	Specific References  (2)     |     SL11200R has been referenced in 2 publications. [IF=3.315] Li Z et al. Rapid Differentiation of Multi-Zone Ocular Cells from Human Induced Pluripotent Stem Cells and Generation of Corneal Epithelial and Endothelial Cells. Stem Cells Dev. 2019 Mar 5.  IF ;  Human.   PubMed:30712489 [IF=1.36] Geng, Rongqing, et al. "Cyclic expression of Lhx2 is involved in secondary hair follicle development in cashmere goat." Gene Expression Patterns (2014).  IHC-P ;  Goat.   PubMed:25128627
Research Area	Neurobiology  Stem cells  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	45kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human LHX2: 251-330/406
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	During development, genetically distinct subtypes of motor neurons express unique combinations of LIM-type homeodomain factors, which regulate cell migration and guide motor axons to establish the fidelity of a binary choice in axonal trajectory. The LIM gene family encodes a set of gene products, which carry the LIM domain, a unique cysteine-rich zinc-binding domain. At least 40 members of this family have been identified in vertebrates and invertebrates, and are distributed into 4 groups according to the number of LIM domains and to the presence of homeodomains and kinase domains. The overlapping expression of LHX1, LHX3, LHX4, Isl-1 and Isl-2 in developing motor neurons along the spinal column may influence the establishment of specific motor neuron subtypes. The human LHX2 gene maps to chromosome 9q33.3 and encodes a 389 amino acid protein. LHX2 is involved in early patterning of the telencephalon, where the neuroepithelium is first divided into cortical tissue and cortical hem. Function: Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types. Subunit: Contains 1 homeobox DNA-binding domain. Contains 2 LIM zinc-binding domains. Subcellular Location: Nucleus. Similarity: Contains 1 homeobox DNA-binding domain. Contains 2 LIM zinc-binding omains. SWISS: P50458 Gene ID: 9355 Database links: Entrez Gene: 16870 Mouse Entrez Gene: 296706 Rat SwissProt: Q9Z0S2 Mouse SwissProt: P36198 Rat Unigene: 142856 Mouse Unigene: 81063 Rat
Product Picture	Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Cerebellum tissue lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Rat Cerebellum tissue lysates Lane 5: Human HeLa cell lysates Primary: Anti-LHX2 (SL11200R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kDa Observed band size: 47 kDa Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LHX2) Polyclonal Antibody, Unconjugated (SL11200R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).

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