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Product Name VGLUT1/BNP1 Chinese Name 脑特异性钠依赖无机磷Transporter抗体 Alias BNPI; Brain specific Na (+) dependent inorganic phosphate cotransporter; Brain specific Na dependent inorganic phosphate cotransporter; Brain-specific Na(+)-dependent inorganic phosphate cotransporter; Slc17a7; Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7; Solute carrier family 17 member 7; Vesicular glutamate transporter 1; VGLU1_HUMAN; VGluT1. literatures Specific References (4) | SL11167R has been referenced in 4 publications.[IF=9.995] Qian Fang. et al. YTHDF1 phase separation triggers the fate transition of spermatogonial stem cells by activating the IκB-NF-κB-CCND1 axis. CELL REP. 2023 Apr 14;42(4):112403 IF ; Mouse.[IF=2.11] Fujita, Yu, et al. "Expression of MEGF10 in cholinergic and glutamatergic neurons." Neuroscience Letters (2017). IHC ; Mouse.[IF=1.931] Wan, Dong-Feng. et al. Late Exercise Preconditioning Regulates BNP Increasing to Assist the Cardioprotection via Up-Regulation of NPR-A and Down-Regulation of NPR-C in Rat Myocardium. Int J Pept Res Ther. 2022 Jan;28(1):1-11 WB,IHC ; Rat.[IF=0] Canhong Wang. et al. Agarwood essential oil inhalation exerts antianxiety and antidepressant effects via the regulation of Glu/GABA system homeostasis. BIOMED REP. 2023 Feb;18(2):1-11 WB ; Mouse.Research Area Neurobiology Signal transduction Cell adhesion molecule Cytoskeleton The cell membrane蛋白 Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 62kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human VGLUT1/BNP1: 301-400/560 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail BNPI; Brain specific Na (+) dependent inorganic phosphate cotransporter; Brain specific Na dependent inorganic phosphate cotransporter; Brain-specific Na(+)-dependent inorganic phosphate cotransporter; Slc17a7; Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7; Solute carrier family 17 member 7; Vesicular glutamate transporter 1; VGLU1_HUMAN; VGluT1.
Function:
Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
Subcellular Location:
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane; Multi-pass membrane protein (Potential). Cell junction, synapse, synaptosome.
Tissue Specificity:
Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe.
Similarity:
Belongs to the major facilitator superfamily.
Sodium/anion cotransporter family. VGLUT subfamily.
SWISS:
Q9P2U7
Gene ID:
57030
Database links:
Entrez Gene: 57030 Human
Entrez Gene: 72961 Mouse
Omim: 605208 Human
SwissProt: Q9P2U7 Human
SwissProt: Q3TXX4 Mouse
Unigene: 375616 Human
Unigene: 255631 Mouse
Unigene: 10267 Rat
Product Picture Sample:
Lane 1: Rat Cerebrum tissue lysates
Lane 2: Human SH-SY5Y cell lysates
Lane 3: Human MCF-7 cell lysates
Primary: Anti-VGLUT1/BNP1 (SL11167R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 60 kDa
Tissue/cell: human gliomas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-VGLUT1/BNP1 Polyclonal Antibody, Unconjugated(SL11167R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-VGLUT1/BNP1 Polyclonal Antibody, Unconjugated(SL11167R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VGLU1) Polyclonal Antibody, Unconjugated (SL11167R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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