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Product Name VAT1L Chinese Name 突触小泡膜蛋白同源样蛋白1抗体 Alias VAT1L_HUMAN; Synaptic vesicle membrane protein VAT-1 homolog-like; EC:1.-.-.- ; KIAA1576; vesicle amine transport 1 like; Research Area Tumour Cell biology immunology Neurobiology Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Pig, Cow, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 46kDa Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human VAT1L: 321-419/419 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Predicted to enable oxidoreductase activity and zinc ion binding activity. [provided by Alliance of Genome Resources, Apr 2022]
Similarity:
Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
SWISS:
Q9HCJ6
Gene ID:
57687
Database links:Entrez Gene: 57687 Human
Entrez Gene: 270097 Mouse
SwissProt: Q9HCJ6 Human
SwissProt: Q80TB8 Mouse
Unigene: 461405 Human
Unigene: 334825 Mouse
Product Picture Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Rat Cerebellum tissue lysates
Primary: Anti-KIAA1576 (SL11130R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 48 kDa
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIAA1576) Polyclonal Antibody, Unconjugated (SL11130R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KIAA1576) Polyclonal Antibody, Unconjugated (SL11130R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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