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Product Name SP7/Osterix Chinese Name 成骨相关转录因子抗体 Alias SP7_HUMAN; Transcription factor Sp7; OSX; Zinc finger protein osterix; Sp7 transcription factor; Osterix; literatures Specific References (30) | SL1110R has been referenced in 30 publications.Research Area Cell biology Chromatin and nuclear signals Signal transduction Stem cells transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 45kDa Detection molecular weight 45-50/56 kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SP7: 331-431/431 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein is a bone specific transcription factor and is required for osteoblast differentiation and bone formation.[provided by RefSeq, Jul 2010]
Function:
Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences (By similarity).
Subunit:
Interacts with NO66; the interaction is direct and inhibits transcription activator activity.
Subcellular Location:
Nucleus.
Tissue Specificity:
Osteoblast/chondrocyte specific.
DISEASE:
Osteogenesis imperfecta 12 (OI12) [MIM:613849]: A form of osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma. Disease severity ranges from very mild forms without fractures to intrauterine fractures and perinatal lethality. Extraskeletal manifestations, which affect a variable number of patients, are dentinogenesis imperfecta, hearing loss, and blue sclerae. OI12 is an autosomal recessive form characterized by recurrent fractures, mild bone deformations, generalized osteoporosis, delayed teeth eruption, no dentinogenesis imperfecta, normal hearing, and white sclerae. Note=The disease is caused by mutations affecting the gene represented in this entry.
Similarity:
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
SWISS:
Q8TDD2
Gene ID:
121340
Database links:Entrez Gene: 121340 Human
Entrez Gene: 170574 Mouse
Omim: 606633 Human
SwissProt: Q8TDD2 Human
SwissProt: Q5RM08 Mouse
SwissProt: Q8VI67 Mouse
Osterix成骨相关转录因子是一种具有锌指基序结构域的转录因子,在体内的表达对成骨细胞具有特异性。该蛋白只在发育的骨组织中特异性表达,是成骨Cell differentiation和骨形成过程中所必需的转录因子。骨髓基质Stem cells分化为表达典型的成骨性标志基因的成骨细胞需要OSX的调控。Product Picture Sample:Spinal cord (Mouse) Lysate at 40 ug
Primary: Anti-Osterix(SL1110R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 50kD
Sample:
Lane 1: Mouse Embryo tissue lysates
Lane 2: Mouse Testis tissue lysates
Lane 3: Mouse Cerebrum tissue lysates
Primary: Anti-SP7/Osterix (SL1110R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 47 kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse embryos); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osterix) Polyclonal Antibody, Unconjugated (SL1110R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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