Product Name	PCDH12
Chinese Name	原钙粘附蛋白12/血管内皮钙粘蛋白2抗体
Alias	PCD12_HUMAN; Pcdh12; Protocadherin 12; Protocadherin-12; Vascular cadherin 2; Vascular cadherin-2; Vascular endothelial cadherin 2; Vascular endothelial cadherin-2; VE cadherin 2; VE-cad-2; VE-cadherin-2; VECAD2.
Research Area	Developmental biology  Neurobiology  Signal transduction  Cell adhesion molecule  Cytoskeleton  The cell membrane蛋白
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1μg/Test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	126kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PCDH12: 401-500/1184 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The encoded protein consists of an extracellular domain containing 6 cadherin repeats, a transmembrane domain and a cytoplasmic tail that differs from those of the classical cadherins. The gene localizes to the region on chromosome 5 where the protocadherin gene clusters reside. The exon organization of this transcript is similar to that of the gene cluster transcripts, notably the first large exon, but no significant sequence homology exists. The function of this cellular adhesion protein is undetermined but mouse protocadherin 12 does not bind catenins and appears to have no affect on cell migration or growth. Function: Cellular adhesion molecule that may play an important role in cell-cell interactions at interendothelial junctions. Promotes homotypic calcium dependent aggregation and adhesion and clusters at intercellular junctions. Unable to bind to catenins, weakly associates with the cytoskeleton. Subcellular Location: Cell membrane. Cell junction. Tissue Specificity: Expressed in highly vascularized tissues including the heart and placenta, but most tissues contain a low level of expression. Prominent expression in the spleen. Similarity: Contains 6 cadherin domains. SWISS: Q9NPG4 Gene ID: 51294 Database links: Entrez Gene: 51294 Human Entrez Gene: 116808 Rat Omim: 605622 Human SwissProt: Q9NPG4 Human Unigene: 439474 Human
Product Picture	Sample: SP2/0(Mouse) Cell Lysate at 30 ug Primary: Anti-PCDH12 (SL11109R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 126 kD Observed band size: 126 kD Positive control: (mo)Splenocytes(2% Paraformaldehyde-fixed ) Isotype Control Antibody: Rabbit IgG; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA Secondary Antibody: Goat anti-rabbit IgG-FITC;Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody : Rabbit Anti-CD28 antibody (SL11109R); Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA

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