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Product Name NTN3 Chinese Name 轴突生长诱导因子3抗体 Alias NET3_HUMAN; Netrin-2-like protein; Netrin3; Netrin-3; Netrin 3; NTN2L; NTN3. Research Area Neurobiology Cell adhesion molecule Extracellular matrix Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 59kDa Cellular localization Extracellular matrix Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human NTN3/Netrin-3: 401-500/580 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Netrin proteins are a family of laminin-related secreted proteins that provide guidance signals for axonal growth and cell migration during development. Netrin signaling is dependent on the concentration of calcium outside the cell and the level of PKA activity. In axonal cells, a reduction in PKA activity converts the responsiveness of the axons to the netrin proteins as the cells are repelled, rather than attracted, by the netrin gradient. Neogenin serves as the primary guidance receptor for netrin-3. Netrin-2 and the corresponding mouse homolog netrin-3 are expressed primarily in the lower two-thirds of the spinal cord, and, like netrin-1, they can either attract or repel commissural axons at a distance. Netrin-3 proteins are associated with the axon fibers projecting from motor neurons and from neurons within sympathetic and sensory ganglia, suggesting that netrin-3 may be involved in pathfinding and fasciculation of axon projection. Neogenin serves as the primary guidance receptor for netrin-3. During peripheral nerve development, high netrin-3 expression has been detected in mesenchymal cells, sensory ganglia and muscles. In humans, the gene encoding for the netrin-3 protein is localized to chromosome 16p13.3.
Function:
Netrins control guidance of CNS commissural axons and peripheral motor axons.
Subcellular Location:
Secreted; extracellular space; extracellular matrix.
Tissue Specificity:
Spinal cord.
Similarity:
Contains 3 laminin EGF-like domains.
Contains 1 laminin N-terminal domain.
Contains 1 NTR domain.
SWISS:
O00634
Gene ID:
4917
Database links:Entrez Gene: 4917 Human
Omim: 602349 Human
SwissProt: O00634 Human
Unigene: 158336 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (Human skin carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NTN3) Polyclonal Antibody, Unconjugated (SL11059R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NTN3) Polyclonal Antibody, Unconjugated (SL11059R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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