Product Name	ILVBL
Chinese Name	乙酰乳酸合成酶蛋白抗体
Alias	Acetolactate synthase homolog; Acetolactate synthase like protein; Acetolactate synthase-like protein; AHAS; ILV2H; 209L8; IlvB (bacterial acetolactate synthase) like; IlvB like protein; IlvB-like protein; Ilvbl; ILVBL_HUMAN.
Research Area	Cardiovascular  Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Dog, Pig, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=3ug/Test ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	68kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ILVBL: 301-400/632
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	ILVBL is a 632 amino acid single-pass membrane protein that belongs to the TPP enzyme family. Expressed in the majority of tissues, ILVBL has the highest level of expression in heart, pancreas and placenta. ILVBL is highly homologous to several bacterial enzymes, including the B isozyme of the large catalytic subunit of E. coli acetohydroxy-acid synthase (AHAS) and the oxalyl-coA decarboxylase of O. formigenes, that utilize thiamine pyrophosphate as a cofactor. ILVBL binds one magnesium ion and one thiamine pyrophosphate per subunit, and may catalyze the initial step in branched-chain amino acid biosynthesis. The gene encoding ILVBL maps to human chromosome 19p13.12 and mouse chromosome 10 C1. Subcellular Location: Membrane. Tissue Specificity: Expressed in all tissues tested, with highest expression in heart, pancreas and placenta. Similarity: Belongs to the TPP enzyme family. SWISS: A1L0T0 Gene ID: 10994 Database links: Entrez Gene: 10994 Human Omim: 605770 Human SwissProt: A1L0T0 Human Unigene: 78880 Human
Product Picture	Sample: HepG2 Cell (Human) Lysate at 30 ug Primary: Anti-ILVBL (SL11014R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68kD Observed band size: 68kD Sample: Hcclm3(Human) Cell Lysate at 30 ug MCF-7(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Primary: Anti- ILVBL (SL11014R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-ILVBL antibody (SL11014R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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