Product Name	Urokinase
Chinese Name	尿激酶型纤溶酶原激活因子抗体
Alias	Urokinase; ATF; ATF uPA; PLAU; UPA; Plasminogen activator; Plasminogen activator urinary; Plasminogen activator urokinase; u PA; U plasminogen activator; UPA; URK; Urokinase plasminogen activator; Urokinase type plasminogen activator; Urokinase type plasminogen activator precursor.
literatures	Specific References  (1)     |     SL1098R has been referenced in 1 publications. [IF=5.123] Liu XP et al. A Label-free Photoelectrochemical Biosensor for Urokinase-type Plasminogen Activator Detection Based on a gC 3 N 4/CdS Nanocomposite.Analytica Chimica Acta.1025;99-107.  Photoelectrochemical detection ;   PubMed:10.1016/j.aca.2018.04.051
Research Area	Cell biology  immunology  Neurobiology  Kinases and Phosphatases  Channel protein
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	49kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PLAU: 361-432/432
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Urokinase is a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in the Urokinase gene may be associated with late onset Alzheimer disease and also with decreased affinity for fibrin binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg Val bond in plasminogen. The proprotein is cleaved at a Lys Ile bond by plasmin to form a two chain derivative in which a single disulfide bond connects the amino terminal A chain to the catalytically active, carboxy terminal B chain. This two chain derivative is also called HMW uPA (high molecular weight uPA). HMW uPA can be further processed into LMW uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino terminal fragment. LMW uPA is proteolytically active but does not bind to the uPA receptor. Function: Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. Subcellular Location: Secreted. Tissue Specificity: Expressed in the prostate gland and prostate cancers. Post-translational modifications: Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. Similarity: Belongs to the peptidase S1 family. Contains 1 EGF-like domain. Contains 1 kringle domain. Contains 1 peptidase S1 domain. SWISS: P00749 Gene ID: 5328 Database links: Entrez Gene: 5328 Human Entrez Gene: 18792 Mouse Entrez Gene: 25619 Rat Omim: 191840 Human SwissProt: P00749 Human SwissProt: P06869 Mouse SwissProt: P29598 Rat Unigene: 77274 Human Unigene: 4183 Mouse Unigene: 6064 Rat 尿激酶型纤溶酶激活因子;是一种多效cell factor，参与多种病理生理过程；uPA系统可通过调节纤溶酶的活性降解p淀粉样蛋白;是一种丝氨酸蛋白酶，将血浆酶原转化成血浆酶（纤维蛋白溶酶）。
Product Picture	Sample: Lane 1: Placenta (Mouse) Lysate at 40 ug Lane 2: Liver (Mouse) Lysate at 40 ug Lane 3: Lung (Mouse) Lysate at 40 ug Lane 4: Spleen (Mouse) Lysate at 40 ug Lane 5: Liver (Rat) Lysate at 40 ug Lane 6: Lung (Rat) Lysate at 40 ug Lane 7: Spleen (Rat) Lysate at 40 ug Primary: Anti-Urokinase (SL1098R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40-80 kD Observed band size: 45 kD Sample: Cerebrum (mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti-Urokinase (SL1098R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49kD Observed band size: 48kD Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Urokinase (SL1098R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 49 kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PLAU/UPA/Urokinase Polyclonal Antibody, Unconjugated(SL1098R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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