Product Name	ABI3
Chinese Name	ABI基因家族2抗体
Alias	ABI gene family member 3; Nesh; New molecule including SH3; ABI3_HUMAN.
Research Area	Tumour  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	39kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ABI3: 21-120/366
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	ABI3 is thought to inhibit tumor metastasis. In vitro, ABI3 reduces cell motility. Function: May inhibit tumor metastasis. In vitro, reduces cell motility. Subunit: May interact with PAK1 and PAK2. Probably interacts with TARSH. Subcellular Location: Cytoplasm. Note=Colocalizes with PAK2 at leading edge of cells. Tissue Specificity: Expressed in heart, lung, liver, pancreas, kidney, placenta and at low levels in brain and skeletal muscle. Similarity: Belongs to the ABI family. Contains 1 SH3 domain. SWISS: Q9P2A4 Gene ID: 51225 Database links: Entrez Gene: 51225 Human Entrez Gene: 66610 Mouse Entrez Gene: 303476 Rat Omim: 606363 Human SwissProt: Q9P2A4 Human SwissProt: Q8BYZ1 Mouse Unigene: 130719 Human
Product Picture	Sample: HUVEC(Human) Cell Lysate at 30 ug Primary: Anti-ABI3 (SL10798R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 48 kD Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- ABI3 (SL10798R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 50 kD Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ABI3 Polyclonal Antibody, Unconjugated(SL10798R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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