Product Name	MMP13
Chinese Name	基质金属蛋白酶13抗体
Alias	CLG 3; CLG3; Collagenase 3; Collagenase3; MMP13; MMP 13; MMP-13; Matrix Metalloproteinase 13; MMP 13; MMP13_HUMAN.
literatures	Specific References  (5)     |     SL10581R has been referenced in 5 publications. [IF=5.026] Ting-ting Yu. et al. Deletion at an 1q24 locus reveals a critical role of long noncoding RNA DNM3OS in skeletal development. Cell Biosci. 2021 Dec;11(1):1-15  IF ;  Mouse.   PubMed:33653390 [IF=4.889] Guoqing Li. et al. Teriparatide ameliorates articular cartilage degradation and aberrant subchondral bone remodeling in DMM mice. J ORTHOP TRANSL. 2023 Jan;38:241  IHC ;  Mouse.   PubMed:36514714 [IF=3.923] Gong X et al. Synergistically regulated spontaneous calcium signaling is attributed to cartilaginous extracellular matrix metabolism. (2018) J. Cell. Physiol. Oct 28.  WB ;  Pig.   PubMed:30370672 [IF=3.857] Jin S et al. Sequential Epiphyseal Cartilage Changes of Femoral Heads in C57BL/6 Female Mice Treated with Excessive GlucocorticoidsCartilage.2020 Dec 3;1947603520978574.  WB、IHC ;  Mouse.   PubMed:33269610 [IF=3.386] Tong, Fei. et al. MiR-466d Targeting MMP13 Promotes the Differentiation of Osteoblasts Exposed to a Static Magnetic Field. BIOTECHNOL BIOPROC E. 2023 Feb;:1-10  WB ;  Rat.   PubMed:10.1007/s12257-022-0231-5
Research Area	Tumour  Cardiovascular  Cell biology  Signal transduction  Cytoskeleton  Extracellular matrix
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	52kDa
Cellular localization	Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MMP13: 251-350/471
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]. Function: Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process. Subcellular Location: Secreted, extracellular space, extracellular matrix (Probable). Tissue Specificity: Seems to be specific to breast carcinomas. DISEASE: Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age. Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia Similarity: Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains. SWISS: P45452 Gene ID: 4322 Database links: Entrez Gene: 403763 Dog Entrez Gene: 4322 Human Entrez Gene: 17386 Mouse Entrez Gene: 171052 Rat Omim: 600108 Human SwissProt: P45452 Human SwissProt: P33435 Mouse SwissProt: P23097 Rat Unigene: 2936 Human Unigene: 5022 Mouse Unigene: 10997 Rat
Product Picture	Sample: MG63(Human) Cell Lysate at 30 ug Primary: Anti-MMP13 (SL10581R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 65 kD Sample: Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug Lane 2: Jurkat (Human) Cell Lysate at 30 ug Lane 3: MCF-7 (Human) Cell Lysate at 30 ug Lane 4: Molt-4 (Human) Cell Lysate at 30 ug Lane 5: A431 (Human) Cell Lysate at 30 ug Primary: Anti-MMP13 (SL10581RR) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MMP-13 Polyclonal Antibody, Unconjugated(SL10581R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (rat lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP13) Polyclonal Antibody, Unconjugated (SL10581R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MMP-13 Polyclonal Antibody, Unconjugated(SL10581R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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