Product Name	5HT2A Receptor
Chinese Name	5-羟色胺受体2A抗体
Alias	5HT2A_HUMAN; 5-hydroxytryptamine receptor 2A; HTR2A; HTR2; 5-HT-2; 5-HT-2A; Serotonin receptor 2A;
literatures	Specific References  (4)     |     SL1056R has been referenced in 4 publications. [IF=14.971] Kyritsi K et al. Modulation of the TPH 1/MAO‐A/5 HT/5 HTR 2A/2B/2C Axis Regulates Biliary Proliferation and Liver Fibrosis During Cholestasis. Hepatology.2019 Jul 25.  ICF&IHC ;  Rat&Mouse.   PubMed:doi:10.1002/hep.30880 [IF=3.395] Xiao cui Yuan. et al. Spinal 5-HT2A Receptor is Involved in Electroacupuncture Inhibition of Chronic Pain :. Mol Pain. 0;():  WB ;  Mouse.   PubMed:35240891 [IF=2.72] Lan Yao. et al. Armillaria mellea fermentation liquor ameliorates p-chlorophenylalanine-induced insomnia associated with the modulation of serotonergic system and gut microbiota in rats. 2022 Jan 05  WB,IHC ;  Rat.   PubMed:34984694 [IF=2.59] Sun, Tao, et al. "Antihypertensive effect of formononetin through regulating the expressions of eNOS, 5-HT< sub> 2A/1B receptors and ??< sub> 1-adrenoceptors in spontaneously hypertensive rat arteries."European journal of pharmacology (2013)..  Rat.   PubMed:23123056
Research Area	Neurobiology  Channel protein
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-F=1:100-500 IF=1:200-800 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	52kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human 5HT2A Receptor: 65-160/471 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	5HT2A receptor is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. 5HT2A receptor belongs to the G-protein coupled receptor 1 family and mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production. 5HT2A receptor is an integral membrane protein which localizes to the post-synaptic thickening of axo-dendritic synapses. 5HT2A receptor protein contains a PDZ domain-binding motif which is involved in the interaction with INADL, CASK, APBA1, DLG1 and DLG4. Function: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production. Subunit: Interacts with MPDZ and INADL. May interact with MPP3, PRDX6, DLG4, DLG1, CASK, APBA1 and MAGI2. Subcellular Location: Cell membrane; Multi-pass membrane protein. Note=Localizes to the postsynaptic thickening of axo-dendritic synapses. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P28223 Gene ID: 3356 Database links: Entrez Gene: 3356 Human Entrez Gene: 15558 Mouse Entrez Gene: 29595 Rat Omim: 182135 Human SwissProt: P28223 Human SwissProt: P35363 Mouse SwissProt: P14842 Rat Unigene: 654586 Human Unigene: 72630 Human Unigene: 214351 Mouse Unigene: 10294 Rat Unigene: 146756 Rat
Product Picture	Sample: U251(Human) Cell Lysate at 30 ug BV2(Mouse) Cell Lysate at 30 ug Primary: Anti-5HT2A Receptor (SL1056R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-5HT2A Receptor (SL1056R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (SL1056R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (SL1056R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (SL1056R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat lung tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-5HT2A Receptor Polyclonal Antibody, Unconjugated(SL1056R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (SL1056R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G- cy3) for 90 minutes, and DAPI for nuclei staining. Blank control: U-2OS. Primary Antibody (green line): Rabbit Anti-5HT2A Receptor antibody (SL1056R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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