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Product Name Pan Cytokeratin Chinese Name 广谱细胞角蛋白PCK抗体 Alias pan-cytokeratin; pan-CK; pan CK; P-CK; CK-PAN; wide spectrum Cytokeratin; Cytokeratins; [cytokeratins 13, 14, 16, 17, 19, 24]. literatures Specific References (2) | SL10403R has been referenced in 2 publications.[IF=13.801] Napolitano Stefania. et al. Antitumor efficacy of dual blockade with encorafenib plus cetuximab in combination with chemotherapy in human BRAFV600E mutant colorectal cancer.. CLIN CANCER RES. 2023 Apr;: IHC ; Mouse.[IF=2.6] Niu YN, Wang K, Jin S, Fan DD, Wang MS, Xing NZ, Xia SJ. "The intriguing role of fibroblasts and c-Jun in the chemopreventive and therapeutic effect of finasteride on xenograft models of prostate cancer." Asian J Androl IHC-P ; Mouse.Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Dog, Cow, Horse, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human cytokeratin 16 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Antibody to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
Subcellular Location:
Cytoplasmic.
SWISS:
P13646
Gene ID:
Pan Cytokeratin
Database links:CK13:
Entrez Gene: 3860 Human
Omim: 148065 Human
SwissProt: P13646 Human
Unigene: 654550 Human
CK14:
Entrez Gene: 3861 Human
Omim: 148066 Human
SwissProt: P02533 Human
Unigene: 654380 Human
CK16:
Entrez Gene: 3868 Human
Omim: 148067 Human
SwissProt: P08779 Human
Unigene: 655160 Human
CK17:
Entrez Gene: 3872 Human
Omim: 148069 Human
SwissProt: Q04695 Human
Unigene: 2785 Human
CK19:
Entrez Gene: 3880 Human
Omim: 148020 Human
SwissProt: P08727 Human
Unigene: 654568 Human
CK24:
Entrez Gene: 192666 Human
Omim: 607742 Human
SwissProt: Q2M2I5 Human
Unigene: 87383 Human
Product Picture Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- Pan Cytokeratin Polyclonal Antibody, Unconjugated(SL10403R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- Pan Cytokeratin Polyclonal Antibody, Unconjugated(SL10403R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Pan Cytokeratin) Polyclonal Antibody, Unconjugated (SL10403R) at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin (SL0437P-Cy3) at[1:500] for 40 minutes and DAPI staining of the nuclei.
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