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Product Name Cytokeratin 19 Chinese Name 细胞角蛋白19抗体 Alias 40 kDa keratin intermediate filament; CK 19; ck19; CK19; CK-19; KRT19; Cytokeratin 19; Cytokeratin-19; k19; k1cs; Keratin 19; Keratin-19; Keratin type I 40 kD; Keratin type i 40kD; Keratin type I cytoskeletal 19; krt19; mgc15366; K1C19_HUMAN; Keratin, type I cytoskeletal 19; Keratin, type I, 40 kd. literatures Specific References (4) | SL1028R has been referenced in 4 publications.[IF=5.116] Jian Liu. et al. Involvement of miRNA203 in the proliferation of epidermal stem cells during the process of DM chronic wound healing through Wnt signal pathways. Stem Cell Res Ther. 2020 Dec;11(1):1-10 IHC ; Rat.[IF=3.267] Joung-Hee Kim. et al. Mealworm Oil (MWO) Enhances Wound Healing Potential through the Activation of Fibroblast and Endothelial Cells. Molecules. 2021 Jan;26(4):779 IHC ; Rat.[IF=2.24] Yang Rong-Hua, et al. An improved method for the isolation and culture of rat epidermal stem cells. International Journal of Clinical and?Experimental Pathology6.11 (2013): 2529-2534. ICC ; Rat.[IF=0] Yunsheng Wang. et al. A rare case of giant panda cancer: Pancreatic ductal adenocarcinoma. Animal Models and Experimental Medicine. 2022 Nov;: IHC ; Panda.Research Area Tumour Cell biology Stem cells Cell type markers Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Rat, ) Applications WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 44kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CK19: 168-270/400 <Cytoplasmic> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]
Function:
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Subunit:
Heterotetramer of two type I and two type II keratins. Interacts with PNN and the actin-binding domain of DMD. Interacts with HCV core protein.
Tissue Specificity:
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P08727
Gene ID:
3880
Database links:Entrez Gene: 3880 Human
Entrez Gene: 16669 Mouse
Omim: 148020 Human
SwissProt: P08727 Human
SwissProt: P19001 Mouse
Unigene: 654568 Human
Unigene: 439699 Mouse
结构蛋白(Structural Proteins)
CK-19细胞角蛋白-19(又称CYFRa21-1)主要用于Tumour方面的研究,近年来大多用于消化系统恶性Tumour的鉴别,有学者认为:CK-19在胰腺Stem cells分化过程中有细胞角蛋白19的表达,把细胞角蛋白19作为胰腺Stem cells的Maker。阳性部位:主要在胞浆。Product Picture Blank control(black line):MCF-7.
Primary Antibody (green line): Rabbit Anti-Cytokeratin 19 antibody (SL1028R)
Dilution:1ug/Test;
Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control(orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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