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Product Name SYF2 Chinese Name SYF2蛋白抗体 Alias Pre-mRNA-splicing factor SYF2; CCNDBP1-interactor; p29; SYF2_HUMAN. Research Area Cell biology immunology Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 29kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SYF2: 131-230/243 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail SYF2 is a nuclear protein that interacts with cyclin D-type binding-protein 1, which is thought to be a cell cycle regulator at the G1/S transition. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Function:
May be involved in pre-mRNA splicing (By similarity).
Subunit:
Identified in the spliceosome C complex. Interacts with CCNDBP1.
Subcellular Location:
Nucleus.
Tissue Specificity:
Abundantly expressed in the heart, skeletal muscle and kidney. Expressed at lower level other tissues.
Similarity:
Belongs to the SYF2 family.
SWISS:
O95926
Gene ID:
25949
Database links:Entrez Gene: 25949 Human
Entrez Gene: 68592 Mouse
Omim: 607090 Human
SwissProt: O95926 Human
SwissProt: Q9D198 Mouse
Unigene: 20013 Human
Unigene: 29989 Mouse
Unigene: 162117 Rat
Product Picture Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SYF2 Polyclonal Antibody, Unconjugated(SL10290R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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