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Rabbit Anti-FAR2 antibody
Rabbit Anti-FAR2 antibody
Fatty acyl CoA reductase 2; FLJ10462; Male sterility domain containing protein 1; MLSTD1; FACR2_HUMAN; FAR2.
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  • NO.:SL10256R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,)
    Applications:WB ELISA IHC-P IHC-F ICC IF
    concentration:1mg/ml
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Details

Product Name FAR2
Chinese Name 脂肪酰辅酶A还原酶2抗体
Alias Fatty acyl CoA reductase 2; FLJ10462; Male sterility domain containing protein 1; MLSTD1; FACR2_HUMAN; FAR2.  
Research Area Tumour  Cardiovascular  Cell biology  Signal transduction  transcriptional regulatory factor  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, )
Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 57kDa
Cellular localization The nucleus cytoplasmic 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human FAR2: 51-150/515 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail This gene belongs to the short chain dehydrogenase/reductase superfamily. It encodes a reductase enzyme involved in the first step of wax biosynthesis wherein fatty acids are converted to fatty alcohols. The encoded peroxisomal protein utilizes saturated fatty acids of 16 or 18 carbons as preferred substrates. Alternatively spliced transcript variants have been observed for this gene. Related pseudogenes have been identified on chromosomes 2, 14 and 22. [provided by RefSeq, Nov 2012]

Function:
FAR2 catalyzes the reduction of fatty acyl-CoA to fatty alcohols. The preferred substrates are C16, C18, C18:1 and C18:2 but low activity can be observed with C10-C14 substrates.

Subcellular Location:
Peroxisome membrane; Multi-pass membrane protein (Potential). Endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Note=Peroxisome in cells expressing low levels of the protein. Peroxisome and endoplasmic reticulum in cells expressing high levels of the protein.

Similarity:
Belongs to the fatty acyl-CoA reductase family.

SWISS:
Q96K12

Gene ID:
55711

Database links:

Entrez Gene: 55711 Human

Entrez Gene: 534380 Cow

Entrez Gene: 330450 Mouse

Entrez Gene: 500368 Rat

SwissProt: Q0P5J1 Cow

SwissProt: Q96K12 Human

SwissProt: Q7TNT2 Mouse



Product Picture
Sample:
HL-60 (Human) Cell Lysate at 30 ug
Primary: Anti-FAR2 (SL10256R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
Sample:
U937 (Human) Cell Lysate at 30 ug
Primary: Anti-FAR2 (SL10256R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDNF) Polyclonal Antibody, Unconjugated (SL10256R FAR2) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FAR2) Polyclonal Antibody, Unconjugated (SL10256R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FAR2) Polyclonal Antibody, Unconjugated (SL10256R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FAR2) Polyclonal Antibody, Unconjugated (SL10256R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FAR2) Polyclonal Antibody, Unconjugated (SL10256R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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