Product Name	CRHR1
Chinese Name	促肾上腺皮质释放激素受体1抗体
Alias	CRF1; Corticotropin releasing hormone receptor variant 1h;CRF-RI; CRF 1; CRF R; CRFR1; CRH R1h; CRF-R1; CRHR 1; CRHR; CRHR1f; Releasing hormone receptor 1; Seven transmembrane helix receptor; corticotropin releasing hormone receptor 1; Crhr; MGC124237; MGC124240; CRF-R; CRF-R-1; CRF-R1; CRFR-1; CRH-R-1; CRH-R1; CRH-R1h; CRHR; CRHR1L; CRFR1_HUMAN.
literatures	Specific References  (1)     |     SL10248R has been referenced in 1 publications. [IF=5.273] Hongyu Ma. et al. Corticotropin-releasing factor potentiates glutamatergic input and excitability of presympathetic neurons in the hypothalamus in spontaneously hypertensive rats. NEUROPHARMACOLOGY. 2023 Jun;230:109506  IF ;  Rat.   PubMed:36924924
Research Area	Tumour  Cell biology  immunology  Neurobiology  Growth factors and hormones  Endocrinopathy
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	48kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CRHR1: 55-150/444 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants one of which is a non-coding read-through transcript with the neighboring gene MGC57346. Function: Receptor for corticotropin releasing factor (CRH). Shows high-affinity CRF binding. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Subunit: Interacts (via N-terminal extracellular domain) with CRF and UCN. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Predominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe. Post-translational modifications: C-terminal Ser or Thr residues may be phosphorylated. Similarity: Belongs to the G-protein coupled receptor 2 family. SWISS: P34998 Gene ID: 1394 Database links: Entrez Gene: 1394 Human Entrez Gene: 12921 Mouse Entrez Gene: 58959 Rat Omim: 122561 Human SwissProt: P34998 Human SwissProt: P35347 Mouse SwissProt: P35353 Rat Unigene: 417628 Human Unigene: 1892 Mouse Unigene: 10499 Rat
Product Picture	Sample: muscle (mouse) Lysate at 40 ug Primary: Anti-CRHR1(SL10248R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 58 kD Sample: kidney (mouse) Lysate at 40 ug Primary: Anti-CRHR1(SL10248R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 58 kD Sample: heart (mouse) Lysate at 40 ug Primary: Anti-CRHR1(SL10248R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 58 kD Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Cerebellum (Mouse) Lysate at 40 ug Lane 3: Testis (Mouse) Lysate at 40 ug Lane 4: Cerebrum (Rat) Lysate at 40 ug Lane 5: Cerebellum (Rat) Lysate at 40 ug Primary: Anti-CRHR1 (SL10248R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50-55 kD Observed band size: 48 kD Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min; Incubation: Anti-CRHR1 Polyclonal Antibody, Unconjugated(SL10248R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (SL10248R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (SL10248R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (SL10248R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (SL10248R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (SL10248R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRHR1) Polyclonal Antibody, Unconjugated (SL10248R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Positive control: RSC96 cells Concebtration: 5ug/10^6 cells Incubation conditions: Avoid light , 30 minutes on the ice.

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