TEL: +86 571 56623320 EMAIL: [email protected]
Product Name SECTM1 Chinese Name 分泌和Transmembrane protein1抗体 Alias K12; K12 protein; Protein K-12; Protein K12; SCTM1_HUMAN; Secreted and transmembrane 1; Secreted and transmembrane protein 1; SECTM 1; SECTM1; Type 1a transmembrane protein. literatures Specific References (1) | SL10153R has been referenced in 1 publications.[IF=5.988] Wei Yu. et al. Identification and validation of key biomarkers for the early diagnosis of diabetic kidney disease.. FRONT PHARMACOL. 2022 Aug;13:931282-931282 IHC, WB ; Mouse, Human.Research Area Tumour Cell biology immunology Signal transduction Channel protein TumourCell biologyMaker The cell membrane蛋白 Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 24kDa Cellular localization The cell membrane Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SECTM1: 42-150/248 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Detected at the highest levels in peripheral blood leukocytes and breast cancer cell lines. Found in leukocytes of the myeloid lineage, with the strongest expression observed in granulocytes and no detectable expression in lymphocytes. Expressed in thymic epithelial cells and fibroblasts.Belongs to the SECTM family.
Function:
May be involved in thymocyte signaling.
Subunit:
Interacts with CD7.
Subcellular Location:
Cell membrane. Secreted.
Tissue Specificity:
Detected at the highest levels in peripheral blood leukocytes and breast cancer cell lines. Found in leukocytes of the myeloid lineage, with the strongest expression observed in granulocytes and no detectable expression in lymphocytes. Expressed in thymic epithelial cells and fibroblasts.
DISEASE:
Detected at the highest levels in peripheral blood leukocytes and breast cancer cell lines. Found in leukocytes of the myeloid lineage, with the strongest expression observed in granulocytes and no detectable expression in lymphocytes. Expressed in thymic epithelial cells and fibroblasts.
Similarity:
Belongs to the SECTM family.
SWISS:
Q8WVN6
Gene ID:
6398
Database links:Entrez Gene: 6398 Human
Omim: 602602 Human
SwissProt: Q8WVN6 Human
Unigene: 558009 Human
Product Picture Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SECTM1 Polyclonal Antibody, Unconjugated(SL10153R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (rat live); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SECTM1) Polyclonal Antibody, Unconjugated (SL10153R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SECTM1) Polyclonal Antibody, Unconjugated (SL10153R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (pv-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SECTM1) Polyclonal Antibody, Unconjugated (SL10153R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Scan Wechat Qrcode