Product Name	Nesfatin-1
Chinese Name	新的饱食分子蛋白抗体
Alias	DNA binding protein NEFA; DNA-binding protein NEFA; Gastric cancer antigen Zg4; NEFA; Nefastin 1; Nefastin-1; Nefastin1; Nucb2; NUCB2_HUMAN; Nucleobindin-2; Nucleobinding 2.
Research Area	Cardiovascular  Cell biology  immunology  Neurobiology  Growth factors and hormones  Diabetes  Binding protein
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, Dog, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	10/47kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Nesfatin-1: 31-130/420
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011]. Function: Calcium-binding protein. May have a role in calcium homeostasis. Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance. Subcellular Location: Golgi apparatus. Membrane; Peripheral membrane protein. Cytoplasm. Secreted. Endoplasmic reticulum. Nucleus envelope. Note=Golgi retention is mediated by its N-terminal region. Tissue Specificity: Predominantly expressed in spleen, testis and normal stomach. Similarity: Belongs to the nucleobindin family. Contains 2 EF-hand domains. SWISS: P80303 Gene ID: 4925 Database links: Entrez Gene: 4925 Human Entrez Gene: 53322 Mouse Entrez Gene: 59295 Rat Omim: 608020 Human SwissProt: P80303 Human SwissProt: P81117 Mouse SwissProt: Q9JI85 Rat Unigene: 654599 Human Unigene: 9901 Mouse Unigene: 41602 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nesfatin-1) Polyclonal Antibody, Unconjugated (SL10068R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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