Product Name	EPHA10
Chinese Name	酪氨酸蛋白激酶受体A10抗体
Alias	EPH receptor A10; EPHA 10; Epha10; EphA10s protein; EPHAA_HUMAN; Ephrin type A receptor 10; Ephrin type-A receptor 10.
literatures	Specific References  (2)     |     SL10052R has been referenced in 2 publications. [IF=5.714] Wenyue Zhao. et al. EphA10 drives tumor progression and immune evasion by regulating the MAPK/ERK cascade in lung adenocarcinoma. INT IMMUNOPHARMACOL. 2022 Sep;110:109031  WB, IHC ;  Human.   PubMed:35839564 [IF=4.32] Zang et al. Anti-EphA10 antibody-conjugated pH-sensitive liposomes for specific intracellular delivery of siRNA. (2016) Int.J.Nanomedicin. 11:3951-67  FCM(FACS) ;  Human.   PubMed:27574425
Research Area	Cell biology  Signal transduction  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	107kDa
Cellular localization	The cell membrane Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human EPHA10: 151-250/1008 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The Eph subfamily represents the largest group of receptor protein tyrosine kinases identified to date (1–3). While the biological activities of these receptors have yet to be determined, there is increasing evidence that they are involved in central nervous system function and in development (1–3). The Eph subfamily receptors of human origin (and their murine/avian homologs) include EphA1 (Eph), EphA2 (Eck), EphA3 (Hek4), EphA4 (Hek8), EphA5 (Hek7), EphA6 (Hek12), EphA7 (Hek11/MDK1), EphA8 (Hek3), EphB1 (Hek6), EphB2 (Hek5), EphB3 (Cek10, Hek2), EphB4 (Htk), EphB5 (Hek9) and EphB6 (Mep). Ligands for Eph receptors include ephrin-A4 (LERK-4) which binds EphA3 and EphB1. In addition, ephrin-A2 (ELF-1) has been described as the ligand for EphA4, ephrin-A3 (Ehk1-L) as the ligand for EphA5 and ephrin-B2 (Htk-L) as the ligand for EphB4 (Htk) (4–7). Function: Receptor for members of the ephrin-A family. Binds to EFNA3, EFNA4 and EFNA5. Subcellular Location: Isoform 1, 3: Cell membrane; Single-pass type I membrane protein (Probable). Isoform 2: Secreted (Probable). Tissue Specificity: Mainly expressed in testis. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 1 Eph LBD (Eph ligand-binding) domain. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain. SWISS: Q5JZY3 Gene ID: 284656 Database links: Entrez Gene: 284656 Human Entrez Gene: 230735 Mouse Entrez Gene: 298528 Rat Omim: 611123 Human SwissProt: Q5JZY3 Human SwissProt: Q8BYG9 Mouse Unigene: 129435 Human Unigene: 171490 Mouse Unigene: 484372 Mouse
Product Picture	Sample: Testis (Mouse) Lysate at 40 ug Testis (Rat) Lysate at 40 ug K562(Human) Cell Lysate at 30 ug Primary: Anti- EPHA10 (SL10052R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 107 kD Observed band size: 107 kD Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-EPHA10 Polyclonal Antibody, Unconjugated(SL10052R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat testis tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-EPHA10 Polyclonal Antibody, Unconjugated(SL10052R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei

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