Product Name	EAAT1
Chinese Name	胶质细胞谷氨酸运载蛋白1抗体
Alias	EA6; EAAT1; Excitatory amino acid transporter 1; FLJ25094; GLAST; GLAST1; Glial high affinity glutamate transporter; High affinity neuronal glutamate transporter; Slc1a3; Sodium dependent glutamate/aspartate transporter; EAA1_HUMAN.
literatures	Specific References  (4)     |     SL1003R has been referenced in 4 publications. [IF=4.879] Tomasz Stępień. et al. Neuropathological Changes in the Brains of Suicide Killers. Biomolecules. 2021 Nov;11(11):1674  IHC ;  Human.   PubMed:34827673 [IF=4.721] Ohmichi T et al.Quantification of brain-derived extracellular vesicles in plasma as a biomarker to diagnose Parkinson's and related diseases.(2018)Parkinsonism Relat Disord. Nov 20.  ELISA ;  Human.   PubMed:30502924 [IF=1.06] Xiao, Ke-Qing, et al. "Effect of subarachnoid nerve block anesthesia on glutamate transporter GLAST and GLT-1 expressions in rabbits." Asian Pacific Journal of Tropical Medicine (2015).  IHC-P ;  Rabbit.   PubMed:26276289 [IF=0.66] Lu, Jun, et al. "Expression of GFAP, GLAST and GLT-1 in astrocytes in patients with mesial temporal lobe epilepsy." Journal of Hainan Medical University 22.2 (2016): 87-90.  IHC-P ;  Human.   PubMed:notpostedyet
Research Area	Neurobiology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	60kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human EAAT1: 301-400/512
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014] Function: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Subcellular Location: Membrane; Multi-pass membrane protein Tissue Specificity: Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. Post-translational modifications: Glycosylated. DISEASE: Defects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia. Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. SWISS: P43003 Gene ID: 6507 Database links: Entrez Gene: 6507 Human Entrez Gene: 20512 Mouse Entrez Gene: 29483 Rat Omim: 600111 Human SwissProt: P43003 Human SwissProt: P56564 Mouse SwissProt: P24942 Rat Unigene: 481918 Human Unigene: 204834 Mouse Unigene: 34134 Rat EAAT-1主要用于神经胶质细胞的损伤及退变方面的研究.
Product Picture	Sample: Lane 1: U87MG (Human) Cell Lysate at 30 ug Lane 2: U251 (Human) Cell Lysate at 30 ug Primary: Anti-EAAT1 (SL1003R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD Tissue/cell: rat brain tissue(Glial cells); 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NPY/Neuropeptide Y Polyclonal Antibody, Unconjugated(SLR) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EAAT1) Polyclonal Antibody, Unconjugated (SL1003R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EAAT1) Polyclonal Antibody, Unconjugated (SL1003R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control: U87MG. Primary Antibody (green line): Rabbit Anti-EAAT1 antibody (SL1003R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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