Product Name	MGMT
Chinese Name	O6甲基鸟嘌呤DNA甲基转移酶抗体
Alias	6 O methylguanine DNA methyltransferase; Agat; AGT; AI267024; EC 2.1.1.63; Methylated DNA protein cysteine methyltransferase; Methylguanine DNA methyltransferase; MGC107020; MGMT: O-6 methylguanine DNA methyltransferase; O 6 methylguanine DNA alkyltransferase; O 6 methylguanine DNA methyltransferase; O-6 methylguanine DNA alkyltransferase; O-6-methylguanine-DNA methyltransferase; O6 methylguanine DNA methyltransferase; MGMT_HUMAN.
literatures	Specific References  (3)     |     SL1002R has been referenced in 3 publications. [IF=3.61] Oláh, Zita, et al. "Proteomic Analysis of Cerebrospinal Fluid in Alzheimers Disease: Wanted Dead or Alive." Journal of Alzheimers Disease (2014).  WB ;  Human.   PubMed:25428253 [IF=2.98] Li et al. Effects of low-intensity ultrasound combined with low-dose carboplatin in an orthotopic hamster model of tongue cancer: A preclinical study. (2018) Oncol.Rep. 39:1609-1618  IHC,WB ;  Hamsters.   PubMed:29436690 [IF=2.886] Yusi Liu. et al. Synergistic Effects of Resveratrol and Temozolomide Against Glioblastoma Cells: Underlying Mechanism and Therapeutic Implications. Cancer Manag Res. 2020; 12: 8341–8354  ICC ;  Rat, Human.   PubMed:32982428
Research Area	Tumour  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, )
Applications	IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	22kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MGMT: 101-207/207
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	MGMT (O6 methylguanine DNA methyltransferase) is an enzyme that transfers methyl groups from O(6) methylguanine, and other methylated moities of DNA, to a cysteine residue in itself, thus repairing DNA damage caused by alkylation in a single step reaction. This is a suicide reaction as the enzyme is irreversibly inactivated. MGMT prevents mutagenesis and malignant transformation, and also provides resistance to chemotherapy with alkylating agents among cancer patients. Function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Subcellular Location: Nucleus. Similarity: Belongs to the MGMT family. SWISS: P16455 Gene ID: 4255 Database links: Entrez Gene: 4255 Human Omim: 156569 Human SwissProt: P16455 Human Unigene: 501522 Human O-6-methylguanine-DNA methyltransferase(MGMT)O-6-甲基鸟嘌呤-DNA-甲基转移酶:是广泛存在于细菌到哺乳动物机体中的一种独特DNA修复酶，MGMT是一种DNA修复酶，是突变和癌变的抑制因子。它对烷化剂导致的DNA损伤有修复作用。 MGMT是突变和癌基因的抑制因子。研究表明MGMT水平与恶性Tumour的发生、进展、浸润、预后及治疗有密切关系。细胞中MGMT水平与其所能耐受的DNA损伤密切相关。一般来讲，低水平表达MGMT的Tumour可以应用烷化剂药物治疗；反之则为耐药。在正常组织中，肝脏高表达；脑组织和骨髓低水平表达。
Product Picture	Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MGMT Polyclonal Antibody, Unconjugated(SL1002R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MGMT Polyclonal Antibody, Unconjugated(SL1002R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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