Product Name	CNPase
Chinese Name	2’,3’-环腺苷酸-3’-磷酸二酯酶抗体
Alias	2',3'-cyclic-nucleotide 3'-phosphodiesterase; CNPase; Cnp; Cnp-1; Cnp1; 2''; 2'3' cyclic nucleotide 3' phosphodiesterase; 2'3'-cyclic-nucleotide 3'-phosphodiesterase; 3''-cyclic-nucleotide 3''-phosphodiesterase; CN37_HUMAN; CNP 1; CNP; CNP1.
literatures	Specific References  (3)     |     SL1000R has been referenced in 3 publications. [IF=2.889] Bin Liu. et al. Nicotinamide Improves Cognitive Function in Mice With Chronic Cerebral Hypoperfusion. Front Neurol. 2021; 12: 596641  WB,IHC ;  Mouse.   PubMed:33569040 [IF=2.882] Gul Fatma Yarim. et al. Nobiletin attenuates inflammation via modulating proinflammatory and antiinflammatory cytokine expressions in an autoimmune encephalomyelitis mouse model. Fitoterapia. 2022 Jan;156:105099  IHC ;  Rat.   PubMed:34896483 [IF=2.43] Li, Ping, et al. "CNP signal pathway up-regulated in rectum of depressed rats and the interventional effect of Xiaoyaosan." World Journal of Gastroenterology: WJG 21.5 (2015): 1518.  IHC-P ;  Rat.   PubMed:25663771
Research Area	Neurobiology  Cell type markers
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	48kDa
Cellular localization	cytoplasmic Extracellular matrix
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CNPase: 151-250/421
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Predicted to enable 2',3'-cyclic-nucleotide 3'-phosphodiesterase activity. Involved in substantia nigra development. Located in several cellular components, including extracellular space; microtubule; and plasma membrane. Implicated in hypomyelinating leukodystrophy 20; multiple sclerosis; and schizophrenia. Biomarker of alcoholic liver cirrhosis; multiple sclerosis; and restless legs syndrome. [provided by Alliance of Genome Resources, Nov 2021] Function: May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin. Subunit: Exists as monomers and homodimers. Subcellular Location: Membrane. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Similarity: Belongs to the cyclic nucleotide phosphodiesterase family. SWISS: P09543 Gene ID: 1267 Database links: Entrez Gene: 280752 Cow Entrez Gene: 607694 Dog Entrez Gene: 1267 Human Entrez Gene: 12799 Mouse Entrez Gene: 493772 Pig Entrez Gene: 25275 Rat Omim: 123830 Human SwissProt: P06623 Cow SwissProt: P09543 Human SwissProt: P16330 Mouse SwissProt: P18104 Pig SwissProt: P13233 Rat SwissProt: P56283 Sheep Unigene: 273621 Human Unigene: 15711 Mouse Unigene: 31762 Rat
Product Picture	Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-CNPase(SL1000R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution Predicted band size: 48 kD Observed band size: 46 kD Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-CNPase (SL1000R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48kD Observed band size: 48kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CNPase) Polyclonal Antibody, Unconjugated (SL1000R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CNP/CNPase Polyclonal Antibody, Unconjugated(SL1000R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CNP/CNPase Polyclonal Antibody, Unconjugated(SL1000R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: U-87MG(blue). Primary Antibody:Rabbit Anti-CNPase antibody(SL1000R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (SL1000R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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