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Product Name ET-1 Chinese Name 内皮素-1抗体 Alias Endothelin-1; Big endothelin 1; EDN 1; EDN1; EDN1 protein; Endothelin1; ET 1; ET1; OTTHUMP00000039245; PPET1; Preproendothelin 1; Preproendothelin1. literatures Specific References (9) | SL0954R has been referenced in 9 publications.Research Area Cardiovascular Cell biology immunology Neurobiology Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Sheep, Guinea Pig, ) Applications ELISA=1:5000-20000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 2.5/24kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ET-1(CSCSSLMDKECVYFCHLDIIW Disulfide bridges C1-C15, C3-C11): 53-73/202 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. Endothelin 1 is expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.
Function:
Endothelins are endothelium-derived vasoconstrictor peptides.
Subcellular Location:
Secreted.
Tissue Specificity:
Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.
Similarity:
Belongs to the endothelin/sarafotoxin family.
SWISS:
P05305
Gene ID:
1906
Database links:Entrez Gene: 1906 Human
Entrez Gene: 13614 Mouse
Omim: 131240 Human
SwissProt: P05305 Human
SwissProt: P22387 Mouse
Unigene: 511899 Human
Unigene: 713645 Human
Unigene: 10918 Rat
内皮素(Endothelin-1,ET)是一种强的血管收缩剂,分为ET-1,ET-2,ET-3 三种,ET-1主要在endothelial cells中表达,而ET-2和ET-3则主要在神经系统中表达。ET具有广泛的生物学作用,此抗体是针对内皮素的活性片段(CSCSSLMDKECVYFCHLDIIW Disulfide bridges C1-C15, C3-C11)。Product Picture Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ET-1) Polyclonal Antibody, Unconjugated (SL0954R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ET-1) Polyclonal Antibody, Unconjugated (SL0954R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control:A549.
Primary Antibody (green line): Rabbit Anti-ET-1 antibody (SL0954R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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