Product Name	PAR1
Chinese Name	蛋白酶激活受体-1抗体
Alias	SAR; PAR-1; PAR 1; BXR; CF2R; F2R; HTR; TR; Proteinase-activated receptor 1 precursor; Proteinase activated receptor 1; Thrombin receptor; Coagulation factor II receptor; Prader-Willi/Angelman region-1; Coagulation factor II (thrombin) receptor; PAR1_HUMAN;
literatures	Specific References  (12)     |     SL0828R has been referenced in 12 publications. [IF=4.57] Daisuke Noguchi. et al. The impact of dabigatran treatment on sinusoidal protection against hepatic ischemia‐reperfusion injury in mice. 2020 Oct 27  WB ;  Mouse.   PubMed:33108682 [IF=3.53] Acton, David, and Gareth B. Miles. "Stimulation of Glia Reveals Modulation of Mammalian Spinal Motor Networks by Adenosine." PLOS ONE 10.8 (2015): e0134488.  IHC-P ;  Mouse.   PubMed:26252389 [IF=3.294] Posthuma JJ et al. Protease-activated receptors are potential regulators in the development of arterial endofibrosis in high-performance athletes. (2018) J Vasc Surg.  IHC ;  Human.   PubMed:30314721 [IF=3.161] Shavit Stein E et al. Thrombin Inhibition Reduces the Expression of Brain Inflammation Markers upon Systemic LPS Treatment.Neural Plast. 2018 Jun 19;2018:7692182.  WB ;  Mouse.   PubMed:30018633 [IF=3.01] Liu, Rui, et al. "Identification of FLOT2 as a novel target for microRNA-34a in melanoma." Journal of Cancer Research and Clinical Oncology (2014): 1-14.  WB ;  Human.   PubMed:25403318 [IF=2.81] Shimon et al. Recovery from trauma induced amnesia correlates with normalization of thrombin activity in the mouse hippocampus. (2017) PLoS.One. 12:e0188524  WB ;  Mouse.   PubMed:29182653 [IF=2.8] Shimon, Marina Ben, et al. "Recovery from trauma induced amnesia correlates with normalization of thrombin activity in the mouse hippocampus." PloS one 12.11 (2017): e0188524.  WB ;  Mouse.   PubMed:29182653 [IF=2.454] Thrombin and the Protease-Activated Receptor-1 in Organophosphate-Induced Status Epilepticus V Golderman  WB ;  Mouse.   PubMed:30515700 [IF=2.31] Fan, Hai-Xia, et al. "Expression of MMP-1/PAR-1 and patterns of invasion in oral squamous cell carcinoma as potential prognostic markers." OncoTargets and therapy 8 (2015): 1619.  IHC-P ;  Human.   PubMed:26170698 [IF=2.07] Chao G et al. Teprenone ameliorates diclofenac-induced small intestinal injury via inhibiting protease activated receptors 1 and 2 activityBiomarkers.2021 Feb;26(1):38-44.  IHC ;  Rat.   PubMed:33176506 [IF=1.872] Noguchi D et al. Antiapoptotic Effect by PAR-1 Antagonist Protects Mouse Liver Against Ischemia-Reperfusion Injury. J Surg Res. 2019 Oct 22. pii: S0022-4804(19)30689-4.  WB ;  Mouse&Human.   PubMed:31653415 [IF=1.54] Akaishi T et al. The Synthetic Curcumin Derivative CNB-001 Attenuates Thrombin-Stimulated Microglial Inflammation by Inhibiting the ERK and p38 MAPK Pathways. Biol Pharm Bull. 2020;43(1):138-144.  WB ;  Rat.   PubMed:31902919
Research Area	Tumour  Cell biology  Signal transduction  Kinases and Phosphatases  The cell membrane受体
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	42kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PAR1: 251-350/425 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. [provided by RefSeq, Jul 2008] Function: High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Platelets and vascular endothelial cells. Post-translational modifications: A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand. Phosphorylated; probably mediating desensitization prior to the uncoupling and internalization of the receptor. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P25116 Gene ID: 2149 Database links: Entrez Gene: 2149 Human Omim: 187930 Human SwissProt: P56488 Baboon SwissProt: P25116 Human Unigene: 482562 Human PAR蛋白全称为蛋白酶体活化受体(Protease-activated receptors)，又称：凝血酶受体(Thrombin Receptor)是独立家族的G protein-coupled receptor， 研究认为与炎症，实体Tumour等密切相关。 PAR1蛋白是乳腺癌细胞侵润和迁移的必需因子，它是作为基质金属蛋白酶体1（MMP-1）的受体关键性作用，这一研究结果为研制新的抗癌药物提供新的靶点。
Product Picture	Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti-PAR1 (SL0828R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample: Brain (Rat) Lysate at 30 ug Liver (Mouse) Lysate at 30 ug Primary: Anti- PAR-1 (SL0828R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution; Predicted band size : 42kD Observed band size : 44kD Sample: Lane 1: U2os (Human) Cell Lysate at 30 ug Lane 2: U251 (Human) Cell Lysate at 30 ug Lane 3: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 4: K562 (Human) Cell Lysate at 30 ug Lane 5: A431 (Human) Cell Lysate at 30 ug Primary: Anti-Thrombin receptor (SL0828R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 62 kD Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thrombin receptor PAR1) Polyclonal Antibody, Unconjugated (SL0828R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thrombin receptor/ PAR1) Polyclonal Antibody, Unconjugated (SL0828R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human mammary cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PAR1 Polyclonal Antibody, Unconjugated(SL0828R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PAR1 Polyclonal Antibody, Unconjugated(SL0828R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PAR-1 Polyclonal Antibody, Unconjugated(SL0828R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PAR-1 Polyclonal Antibody, Unconjugated(SL0828R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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